Isolation of DNA-Methyltransferase Genes and Their Promoter Sequences from Strawberry (Fragaria x ananassa Duch.)
文献类型: 会议论文
第一作者: Linlin Chang
作者: Linlin Chang 1 ; Zhihong Zhang 2 ; Yuntao Zhang 1 ; He Li 2 ; Yue Yang 2 ; Hongyan Dai 2 ;
作者机构: 1.Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, No.12A, Ruiwangfen, Haidian District, Beijing 100093, China
2.College of Horticulture, Shenyang Agricultural University, No. 120, Dongling Road, Shenyang, Liaoning 110866, China
关键词: DNA-methyltransferase gene;strawberry;promoter
会议名称: International Horticultural Congress
主办单位:
页码: 85-90
摘要: DNA methylation in plants is required for normal development and plays important roles in regulating gene expression. Patterns of methylation are established by de novo methyltransferase (DRM) and maintained by maintenance methyltransferase (METI) activities. In this study, two putative sequences of DNA methyltransferase genes, FaMET1a and FaDRMa were isolated initially from strawberry (Fragaria xananassa Duch.) using chromosome-walking procedure and rapid-amplificatioh of cDNA ends (RACE). Structural conservation of the amino acid sequence between FaMET1a and PpMET1, and that between FaDRMa and MDRM1 suggested that they could function as DNA methyltransferase. By constructing plasmids with 5' regions from FaMET1a and FaDRMa fused to the coding sequence of a gene encoding β-glucuronidase (GUS), it was demonstrated that the regions upstream of FaMET1a and FaDRMa translational initiation codons contained the 5'-regulatory elements necessary for high-level GUS expression in transiently transformed strawberry fruits.
分类号: S663-532
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