文献类型： 会议论文

第一作者： Zhang Zhi Ping

作者： Zhang Zhi Ping ¹ ; Sheng Ming ¹ ; Li Chui ¹ ; Sun Xin Er ¹ ; Yao Quan Hong ² ; Wang Liang Ju ¹ ;

作者机构： 1.College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China

2.Agro-Biotechnology Research Center, Shanghai Academy of Agricultural Sciences, Shanghai, 201106, China

关键词： 5-aminolevulinic acid (ALA);Arabidopsis thaliana HemAl promoter;Saccharomyces cerevisiae Heml gene;Solanum lycopersicum L.

会议名称： International Horticulture Congress

主办单位：

页码： 305-312

摘要： A gene encoding 5-aminolevulinic acid synthase (ALA-S) in Saccharomyces cerevisiae (Heml) under the control of an Arabidopsis thaliana HemAl promoter (HemAl promoter) was introduced into the genome of tomato (Solanum lycopersicum). All transgenic tomato plants were able to transcribe the Heml gene and the capacity to synthesize ALA was increased. The transgenic plants had 18% higher levels of chlorophylls, as compared to wild-type plants, indicating that part of the additional ALA from yeast ALA-S was metabolized to chlorophylls. Determination of gas exchange suggested that the net photosynthetic rate (P_n) in the transgenic tomato was about 60% higher than that of wild type. The apparent quantum yield (AQY) in the transgenic plants was 0.0363, significantly higher than 0.0313 of wild type. The Chlorophyll-a fast fluorescence measurement and JIP-test at continuous intensities of excitation light (90-2100 μmol m~(-2) s~(-1)) showed that the genetic transformation caused a significant reduction in V_j (the relative variable fluorescence at the J-step) but addition in V_i (the relative variable fluorescence at the I-step). The maximum photochemical efficiency (φp_o=F_v/F_m) did not vary with the light intensity, which demonstrated that φ_(Po) was a stable index for leaf photosynthesis. φ__(Eo) (the probability that a trapped exciton moves an electron into the electron transport chain beyond Q_A) and φ_(Eo) (the quantum yield of electron transport) decreased with the light intensity increased, especially in wild type. Furthermore, the photosynthetic performance index on absorption basis (PI_(ABS)) or cross section basis (PI_(CS)) in the transgenic plants was also increased obviously in transgenic tobacco. Thus, the results obtained in this work approved that ALA improved photosynthetic capacity and the activity of PSII reaction center.

分类号： Q945-532