Studies on the Isolation, Purification of Cellulase Produced by Aspergillus Niger Ⅲ and Trichoderca Viride and their Enzymatic Properties
文献类型： 会议论文
第一作者： Sheng Yang
作者： Sheng Yang 1 ; Hongping Hou 2 ;
作者机构： 1.College of Food Science and Engineering Shanxi Agricultural University The Fruit Trees Science Institute Shanxi Academy of Agricultural Sciences Shanxi China
2.College of Food Science and Engineering Shanxi Agricultural University Shanxi China
关键词： apsergillus niger;trichoderma viride;mixed fermentation;cellulase;isolation and purification
会议名称： International conference on agricultural engineering and food engineering
主办单位：
页码： 1000-1003
摘要： Isolation and purification of the crude cellulase produced by apsergillus niger Ⅲ and trichoderma viride Ⅰ, and the enzymatic properties were studied in the paper. The results: By means of ammonium sulfate salting-out methods and sephadex G-100 column chromatography, five elution peaks were obtained, namely peak 1～5. The peak 2 included endoglucanase and exo-glucanase. Peak3 included endoglucanase, exoglucanase, and B-glycosidase. Peakl, 4 were not active, peak 5 included endoglucanase and Bglycosidase. By DEAE FF weak anion exchange chromatography, a kind of endoglucanase was purified from peak 2, and its molecular weight was 61.5kO by SDS-PAGE cataphoresis. A range of pH 5.0～6.0, the CMCase activity of peak 2 maintained 70%～80% of initial enzymatic activity, and the optimum pH was 5.0;higher enzyme activity appeared at 30～50℃, the optimal temperature was 50℃, and the enzyme activity rapidly decreased when temperature was higher than 60℃.
分类号： s2
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