文献类型： 会议论文

第一作者： Jinbin Wang

作者： Jinbin Wang ¹ ; Dachao Chen ² ; Ziping Zhu ¹ ; Ying Xu ³ ; Xueming Tang ¹ ;

作者机构： 1.Biotechnology Research Institute,Shanghai Academy of Agricultural Sciences,2901 Beidi Road,Shanghai,201106,China

2.Key Laboratory of Agricultural Genetics and Breeding,Shanghai Academy of Agricultural Sciences,2901 Beidi Road,Shanghai,201106,China

3.Shanghai Ocean University,Shanghai,201306,China

关键词： Bacillus subtilis;Electroporation;Transformation efficiency

会议名称： Conference on Environmental Pollution and Public Health

主办单位：

页码： 679-683

摘要： In order to obtain an optimal transformation efficiency of Bacillus subtilis electroporation experimental program,to optimize the conditions for electroporation of Bacillus subtilis cells,a shuttle vector for Escherichia coli and Bacillus subtilis,pHY300PLK,were used.Factors including growth medium,electroporation medium,recovery Medium,plasmid concentration and pulse strength were investigated.Single factor experiments were employed to optimize the growth medium,electroporation medium,recovery Medium,plasmid concentration and pulse strength.Results showed that the optimal values for concentration of the growth medium electroporation medium,recovery Medium,plasmid concentration and pulse strength are LB+0.5 M sorbitol,0.5 M sorbitol+0.5 M mannitol+10％ Glycero,LB+0.5 M sorbitol+0.38 M mannitol,20 kV/ cm and 100 ng/μl ofPlasmid DNA.

分类号： x503.1