Genetic diversity of phytophthoRA sojae isolates in Heilongjiang province in China assessed by RAPD and EST-SSR
文献类型： 会议论文
第一作者： J. J. Wu
作者： J. J. Wu 1 ; P. F. Xu 2 ; L. J. Liu 3 ; J. S. Wang 3 ; W. G. Lin 3 ; S. Z. Zhang 2 ; L. Wei 3 ;
作者机构： 1.Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme, Harbin, China 150086 Soybean Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin, China 150086
2.Soybean Research Institute, Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural University, Harbin, China 150030
3.Soybean Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin, China 150086
关键词： Genetic diversity;Phytophthora sojae;RAPD;EST-SSR;Cluster analysis
会议名称： Conference on intelligent information, control, and communication technology for agricultural engineering
主办单位：
页码： 87620T.1-87620T.4
摘要： Random-amplified polymorphic DNA (RAPD) and EST-SSR markers were used to estimate the genetic relationship among thirty-nine P.sojae isolates from three locations in Heilongjiang Province, and nine isolates from Ohio in America were made as reference strains. 10 of 50 RAPD primers and 5 of 33 EST-SSR were polymorphic across 48 P.sojae isolates. Similarity values among P.sojae isolates were from 49% to 82% based on the RAPD data. The similarities based on EST-SSR markers ranged from 47% to 85%. The genetic diversity revealed by EST-SSR marker analysis was higher than that obtained from RAPD. The similarity matrices for the SSR data and the RAPD data were moderately correlated (r = 0.47). Genetic similarity coefficients were also relatively lower, which demonstrated complicated genetic background within each location. The high similarity values range revealed the ability of RAPD/EST-SSR markers to distinguish even among morphological similar phytophthora.
分类号： tp391
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Genetic diversity of Phytophthora sojae isolates in Heilongjiang Province in China assessed by RAPD and EST-SSR
作者：J. J. Wu;P. F. Xu;L. J. Liu;J. S. Wang;W. G. Lin;S. Z. Zhang;L. Wei
关键词：Genetic diversity;Phytophthora sojae;RAPD;EST-SSR;Cluster analysis
