Polymorphism and Balancing Selection of MHC Class II DAB Gene in 7 Selective Flounder ( Paralichthys olivaceus) Families
文献类型： 会议论文
第一作者： Min Du
作者： Min Du 1 ; Song-lin Chen 3 ; You Liang 3 ; LeiWang 3 ; Feng-tao Gao 3 ; Yang Liu 3 ; Xiao-Lin Liao 3 ;
作者机构： 1.Key Lab for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Nanjing Road 106, Qingdao 266071, China College of Aqua-life Science and Technology,
2.Key Lab for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Nanjing Road 106, Qingdao 266071, China College of Aqua-life Science and Technology,
3.Key Lab for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Nanjing Road 106, Qingdao 266071, China
会议名称： International Marine Biotechnology Conference
主办单位：
页码： 1-10
摘要： @@ In order to determine the genetic variation of the MHC class IIB exon2 allele in the o.spring, 700 fry from seven families of Japanese flounder challenged with V. Anguillarum were studied, and di.erent mortality rates were found in those families. Five to ten surviving and dead fry from each of the seven families were selected to study the MHC class II B exon2 gene with PCR and a direct sequencing method. One hundred and sixteen di.erent exon2 sequences were found and 116 di.erent alleles were identified, while a minimum of four loci were revealed in the MHC class II B exon2 gene. The ratio (dN/dS) of nonsynonymous substitution (dN) to synonymous substitutions (dS) in the peptide-binding region (PBR) of the MHC class IIB gene was 6.234, which indicated that balancing selection is acting on the MHC class IIB genes. The MHC IIB alleles were thus being passed on to their progeny. Some alleles were significantlymore frequent in surviving than dead individuals. All together our data suggested that the alleles Paol-DAB*4301, Paol-DAB*4601, Paol-DAB*4302, Paol-DAB*3803, and Paol-DAB*4101 were associated with resistance to V. Anguillarum in flounder.
分类号： P714.5
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