文献类型： 会议论文

第一作者： Lin CHEN

作者： Lin CHEN ¹ ; Ting BAI ¹ ; Wei WANG ¹ ; Tian-fei LIU ² ; Li-li JI ¹ ; Jia-min ZHANG ¹ ;

作者机构： 1.Key Lab of Meat Processing of Sichuan Province, Chengdu University

2.Institute of Animal Science, Guangdong Academy of Agricultural Sciences

关键词： Clostridium perfringens;Codon usage bias;Correspondence analysis;Optimal codon

会议名称： International Conference on Biological Sciences and Technology

主办单位：

页码： 148-155

摘要： Clostridium perfringens is a conditional pathogen, which widely exists in the nature water, soil, intestinal tract, and serious threat to public health and safety. In this study, the codon usage of Clostridium perfringens was examined. CDS sequences of 2744 high confidence protein coding genes were selected from Clostridium perfringens Genome Database. Their GC content of these genes ranged from 14.7% to 41.9%, with the average of 29.9% for whole genome. The frequency of G or C in the third position of synonymous codon was 25.6%, which was lower than that of A or T. The effective number of codon(ENC) of Clostridium perfringens genome ranged from 26.9 to 61.0, with the average of 39.9. Clostridium perfringens prefer codons ending in A/T. The ENC values of each gene significantly positive correlated with its C3s, G3s, GC3s and GC content (r=0.674, 0.738, 0.780 and 0.368, respectively, p<0.01). However, ENC showed significant negative correlations with T3s, A3s and CAI (r=-0.668, -0.688 and -0.255, respectively, p<0.01). A total of 27 optimal codons were found from Clostridium perfringens genome, all of which the third position of synonymous codon was A or T.

分类号： Q81-53