TREHALOSE SYNTHASE GENE TRANSFER MEDIATED BY AGROBACTERIUM TUMEFACIENS ENHANCES RESISTANCE TO OSMOTIC STRESS IN SUGARCANE
文献类型: 会议论文
第一作者: ZI-ZHANG WANG
作者: ZI-ZHANG WANG 1 ; SHU-ZHEN ZHANG; BEN-PENG YANG; YANG-RUI LI;
作者机构: 1.National Key Biotechnology Laboratory for Tropical Crops, CATAS, Haikou 571101
关键词: agrobacterium tumefaciens;transformation;trehalose synthase gene;sugarcane
会议名称: International Symposium on Sustainable Sugarcane and Sugar Production Technology
主办单位:
页码: 529-534
摘要: Trehalose synthase gene (TSase) from Grifola frondosa was transfered into sugarcane (Saccharum officinarum L.) using Agrobacterium- mediated method to improve sugarcane drought-tolerance. The results indicated that embryogenic callus of sugarcane was sensitive to A. tumefaciens EHA105 strain in the transformation system employed. The high frequency of PPT-resistant plant were obtained from transformated with 3 weeks callus after incubation, which reached 4.5% on average. The transgenic plants was confirmed by PCR and dot-southern analysis. Some trangenic plants showed multiple phenotypic" alterations and some plants demonstrated improvement in tolerance to osmotic stress.
分类号: TS24
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EXPRESSION OF THE GRIFOLA FRONDOSA TREHALOSE SYNTHASE GENE AND IMPROVEMENT OF THE DROUGHT-TOLERANCE IN SUGARCANE
作者:SHU-ZHEN ZHANG;BEN-PENG YANG;CUI-LIAN FENG;RU-KAI CHEN;JING-PING LUO;WEN-WEI CAI;FEI-HU LIU
关键词:Trehalose synthsase gene;Grifola frondosa;Saccharum officinarum L.;drought tolerance