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TREHALOSE SYNTHASE GENE TRANSFER MEDIATED BY AGROBACTERIUM TUMEFACIENS ENHANCES RESISTANCE TO OSMOTIC STRESS IN SUGARCANE

文献类型: 会议论文

第一作者: ZI-ZHANG WANG

作者: ZI-ZHANG WANG 1 ; SHU-ZHEN ZHANG; BEN-PENG YANG; YANG-RUI LI;

作者机构: 1.National Key Biotechnology Laboratory for Tropical Crops, CATAS, Haikou 571101

关键词: agrobacterium tumefaciens;transformation;trehalose synthase gene;sugarcane

会议名称: International Symposium on Sustainable Sugarcane and Sugar Production Technology

主办单位:

页码: 529-534

摘要: Trehalose synthase gene (TSase) from Grifola frondosa was transfered into sugarcane (Saccharum officinarum L.) using Agrobacterium- mediated method to improve sugarcane drought-tolerance. The results indicated that embryogenic callus of sugarcane was sensitive to A. tumefaciens EHA105 strain in the transformation system employed. The high frequency of PPT-resistant plant were obtained from transformated with 3 weeks callus after incubation, which reached 4.5% on average. The transgenic plants was confirmed by PCR and dot-southern analysis. Some trangenic plants showed multiple phenotypic" alterations and some plants demonstrated improvement in tolerance to osmotic stress.

分类号: TS24

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