文献类型： 会议论文

第一作者： SHU-ZHEN ZHANG

作者： SHU-ZHEN ZHANG ¹ ; BEN-PENG YANG; CUI-LIAN FENG; RU-KAI CHEN; JING-PING LUO; WEN-WEI CAI; FEI-HU LIU;

作者机构： 1.State Key Laboratory of Tropical Crop Biotechnology, Chinese Academy of Tropical Agricultural Sciences (CATAS), Haikou 571101, China

关键词： Trehalose synthsase gene;Grifola frondosa;Saccharum officinarum L.;drought tolerance

会议名称： Technologies to improve sugar productivity in developing countries

主办单位：

页码： 606-612

摘要： Trehalose is a nonreducing disaccharide of glucose that functions as a protectant in the stabilization of biological structures and enhances the stress tolerance under abiotic stresses in organisms. We report here the expression of Grifola frondosa trehalose synthase (TSase) gene for improving drought tolerance in sugarcane (Saccharum officinarum L.). The expression of the transgene was under the control of two tandem copies of CaMV 35S promoter and transferred into sugarcane by Agrobacterium tumefaciens EHA105. Compared with nontransgenic plants, the transgenic plants can accumulate high-level products of trehalose, which were increased up to 8.805-12.863mg/ g FW, while it was at undetectable level in nontrasgenic plants. It has been reported thatthe transgenic plants transformed with E.coli TPS and/or TPP were severely stunted and had root morphologic alteration. Interestingly, our transgenic sugarcane plants have no obvious morphological changes and no growth inhibition in the field. Trehaloseaccumulation in 35S-35S: TSase plants resulted in increased drought tolerance to sugarcane, as shown by the detecting results of the tests on drought and the drought physiological indexes, such as the rate of bound water/ free water, plasma membrane permeability (PMP), malondialdehyde content (MDA), chlorophyll a and b content, activity of SOD and POD of the excised leaves. These results suggest that transgenic plants transformed with TSase gene can accumulate high-level of trehalose and enhance the tolerance to drought.

分类号： TS24