文献类型： 会议论文

第一作者： Allan W. Hey

作者： Allan W. Hey ¹ ; Angelo Guidolin; Reza Zareie;

作者机构： 1.BresaGen Limited, 8 Dalgleish Street, Thebarton, South Australia 5031, Australia

会议名称： Understanding Biology Using Peptides

主办单位：

页码： 164-165

摘要： Chemical synthesis is currently seen as the method of choice for production of therapeutic peptides. Production through recombinant bacteria is a viable alternative but is not often pursued due to perceived problems with efficiency and proteolysis. BresaGen has developed a "toolkit" approach for production of peptides as insoluble fusion proteins in E coli. Segregation of peptides into insoluble inclusion bodies allows high expression rates and protects the peptides from proteolysis, while simplifying purification. A variety of fusion partners and cleavage mechanisms allow the system to be customized for the peptide in question, which is important in view of the widely-differing properties of different peptides with therapeutic potential. Certain post-translational modifications such as amidation at the carboxy terminal may also be incorporated.

分类号： O629.7