文献类型： 会议论文
第一作者： Wenpeng Yang
作者： Wenpeng Yang 1 ; Yonglian Zheng; Jing Wu;
作者机构： 1.Institute of Upland Food Crops, Guizhou Academy of Agricultural Sciences and Guizhou Center of Maize Engineering Techniques, Guiyang, Guizhou 550006, P. R. China
关键词： Heterofertilization;Opaque-2;Marker assisted selection;Simple sequence repeat;Unequal crossing over;Maize
会议名称： Proceedings of the ninth Asian regional maize workshop
主办单位：
页码： 294-297
摘要： Selection on endospermous traits among individual kernels is very important in maize because the characteristics of kernels, especially endosperm, are directly related to quality and yield. However the accuracy of it may be affected by heterofertilization. The objective of this work was to investigate the frequency of hetero-fertilization of opaque-2 kernels in maize. Three BCjF, populations using opaque-2 lines as recurrent parents and some BC_1 F_2 progenies were used to investigate endosperm phenotypes and plant genotypes. The plant genotypes were determined using SSR markers, phi057 or phi112, which are sequences located within the opaque-2 allele; the endosperm phenotypes were observed visually. In the BC_1F_1 populations, the frequency of heterofertilization was 0% -4.5%. This result indicates that there may be up to a 5% difference between the selections based on endosperm phenotype and plant genotype in a backcross breeding program. Molecular marker assisted selection should be a more accurate selection procedure. In addition, we discuss the relationship between the endosperm heterofertilization of opaque-2 maize and unequal crossing over within the opaque-2 gene.
分类号： S513
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