文献类型： 会议论文

第一作者： Jerome Gouttenoire

作者： Jerome Gouttenoire ¹ ; Ulrich Valcourt; Marie-Claire Ronziere; Elisabeth Aubert-Foucher; Frederic Mallein-Gerin; Daniel Herbage;

作者机构： 1.Institut de Biologie et Chimie des Proteines, UMR 5086 CNRS-UCB Lyon I, IFR128 BiosciencesLyon-Gerland, 7passage du Vercors, 69367 Lyon CedexO7, France

关键词： Chondiocyte phenotype;type IIA and IIB procollagen;BMP-2;TGF-beta1;cartilage repair

会议名称： Mechanobiology

主办单位：

页码： 535-542

摘要： In osteoarthritic cartilage, chondrocytes are able to present heterogeneous cellular reactions with expression and synthesis of the (pro)collagen types characteristic of prechondrocytes (type IIA), hypertiophic chondrocytes (type X), as well as differentiated (types IIB, IX, XI, VI) and dedifferentiated (types I, III) chondrocytes. The expression of type IIA procollagen in human osteoarthritic cartilage support the assumption that OA chondrocytes reverse their phenotype towards a chondioprogen-itor phenotype. Recently, we have shown that dedifferentiation of mouse chondrocytes induced by subculture was associated with the alternative splicing of type II procollagen pie-mRNA with a switch from the IIB to the IIA form. In this context, we demonstrated that BMP-2 favours expression of type IIB whereas TGF-beta1 potentiates expression of type IIA induced by subculture. These data reveal the specific capability of BMP-2 to reverse the program of chondrocyte dedifferentiation. This interesting feature needs to be tested with human chondrocytes since cell amplification is required for the currently used autol-ogous chondrocyte transplantation.

分类号： R318.01