专利类型： 中国发明申请
专利权人： 中国水产科学研究院珠江水产研究所
发明人： 罗霞;付小哲;李宁求;黄志斌;林强
专利号： CN201510076301.7
摘要： 本发明公开了一种鳜传染性脾肾坏死病毒ISKNV的增殖方法。本发明采用同步接毒的方式，病毒与鳜脑组织细胞系CPB成悬浮状态，互相接触的表面积大，使病毒通过更多的空间受体进入细胞；病毒在细胞分裂增殖的同时侵染细胞，从而获得高含量的ISKNV。当CPB培养30h～60h后，转入5%～8%v/v胎牛血清培养基中，同步接种ISKNV的MOI值为0.85～2.3，得到低成本的ISKNV增殖方法。当CPB培养20h～40h后，转入8%～10%v/v胎牛血清培养基中，同步接种ISKNV的MOI值为30～45，得到短时间的ISKNV增殖方法。可根据现有条件选择培养方法，本发明为低成本疫苗的生产提供了理论依据。
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