61Functional characterization of Class II 5-enopyruvylshikimate-3-phosphate synthase from Halothermothrix orenii H168 in Escherichia coli and transgenic Arabidopsis

作者： Tian, Yong-Sheng; Xu, Jing; Xiong, Ai-Sheng; Zhao, Wei; Gao, Feng; Fu, Xiao-Yan; Peng, Ri-He; Yao, Quan-Hong; SCI

机构： Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai Key Lab Agr Genet & Breeding, Shanghai, Peoples R China;

来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

关键词： 5-Enopyruvylshikimate-3-phosphate synthase Enzyme kinetic values Halothermothrix orenii Transgenic Arabidopsis

62Yeast copper-dependent transcription factor ACE1 enhanced copper stress tolerance in Arabidopsis

作者： Xu, Jing; Tian, Yong-Sheng; Peng, Ri-He; Xiong, Ai-Sheng; Zhu, Bo; Jin, Xiao-Fen; Gao, Jian-Jie; Yao, Quan-Hong; Xu, Jing; Gao, Jian-Jie; Hou, Xi-Lin; SCI

机构： Shanghai Acad Agr Sci, Agrobiotechnol Res Ctr, Shanghai 201106, Peoples R China; Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China;

来源：BMB REPORTS

关键词： ACE1 Arabidopsis Copper tolerance Transgenic plants

63High efficiency and throughput system in directed evolution in vitro of reporter gene

作者： Xiong, Ai-Sheng; Peng, Ri-He; Liu, Jin-Ge; Zhuang, Jing; Qiao, Yu-Shan; Xu, Fang; Cai, Bing; Zhang, Zhen; Chen, Jian-Min; Yao, Quan-Hong; SCI

机构： Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China; Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China; Yangzhou Univ, Coll Biosci & Biotechnol, Yangzhou 225009, Peoples R China; Nanjing Agr Univ, Coll Hort, Nanjing 210095, Peoples R China; Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China; Yangzhou Univ, Coll Biosci &;

来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

关键词： GUS reporter gene DNA directed evolution gene shuffling beta-glucuronidase thermostability GREEN FLUORESCENT PROTEIN BETA-GLUCURONIDASE MOLECULAR EVOLUTION SATURATION MUTAGENESIS SCREENING STRATEGIES RAPID EVOLUTION PLANTS GALACTOSIDASE BIOCATALYSTS ENZYMES

64Mutations in two amino acids in phyI1s from Aspergillus niger 113 improve its phytase activity

作者： Tian, Yong-Sheng; Peng, Ri-He; Xu, Jing; Zhao, Wei; Gao, Feng; Fu, Xiao-Yan; Xiong, Ai-Sheng; Yao, Quan-Hong; SCI

机构： Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai 201106, Peoples R China;

来源：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

关键词： enzyme kinetics substrate binding enzyme catalytic efficiency enzyme structure-function study

65Discovery, phylogeny and expression patterns of AP2-like genes in maize

作者： Zhuang, Jing; Yao, Quan-Hong; Xiong, Ai-Sheng; Deng, De-Xiang; Xiong, Fei; Chen, Jian-Min; Zhuang, Jing; Zhang, Jian; SCI

机构： Shanghai Acad Agr Sci, Shanghai 201106, Peoples R China; Yangzhou Univ, Yangzhou 225009, Peoples R China; Alberta Res Council, Vegreville, AB T9C 1T4, Canada;

来源：PLANT GROWTH REGULATION

关键词： AP2/ERF Arabidopsis thaliana Expression profile Maize Transcription factor

66Advances in directed molecular evolution of reporter genes

作者： Xiong, Ai-Sheng; Xiong, Ai-Sheng; Peng, Ri-He; Yao, Quan-Hong; Zhuang, Jing; Davies, Jim; Zhang, Jian; SCI

机构： Nanjing Agr Univ, Coll Hort, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing, Jiangsu, Peoples R China; Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai, Peoples R China; Alberta Res Council, Alberta Innovates Technol Futures, Vegreville, AB T9C 1T4, Canada;

来源：CRITICAL REVIEWS IN BIOTECHNOLOGY

67A direct and efficient PAGE-mediated overlap extension PCR method for gene multiple-site mutagenesis

作者： Peng, Ri-He; Xiong, Ai-Sheng; Yao, Quan-Hong; SCI

机构： Shanghai Acad Agr Sci, Shanghai Key Lab Agr Genet & Breeding, AgroBiotechnol Res Ctr, Shanghai, Peoples R China;

来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

关键词： POLYMERASE-CHAIN-REACTION ENZYME CATALYSIS DNA MUTATIONS GENERATION PLASMID PRIMER ACID

68Expression and Function of a Modified AP2/ERF Transcription Factor from Brassica napus Enhances Cold Tolerance in Transgenic Arabidopsis

作者： Xiong, Ai-Sheng; Wang, Feng; Jiang, Hai-Hua; Peng, Ri-He; Jin, Xiao-Fen; Zhu, Bo; Yao, Quan-Hong; Zhuang, Jing; Zhang, Jian; SCI

机构： Nanjing Agr Univ, Coll Hort, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China; Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai 201106, Peoples R China; Alberta Innovates Technol Futures Alberta Res Cou, Vegreville, AB T9C 1T4, Canada;

来源：MOLECULAR BIOTECHNOLOGY

关键词： AP2/ERF Arabidopsis Brassica napus Cold stress Directed evolution Transcription factor

69Expression of a rice DREB1 gene, OsDREB1D, enhances cold and high-salt tolerance in transgenic Arabidopsis

作者： Zhang, Yang; Chen, Chen; Jin, Xiao-Fen; Xiong, Ai-Sheng; Peng, Ri-He; Yao, Quan-Hong; Zhang, Yang; Chen, Chen; Hong, Yi-Huan; Chen, Jian-Min; SCI

机构： Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai 201106, Peoples R China; Yangzhou Univ, Coll Biosci & Biotechnol, Yangzhou 225009, Peoples R China;

来源：BMB REPORTS

关键词： Abiotic stress Arabidopsis DREB-type transcription factors Rice Transgenic plants

70Chemical gene synthesis: strategies, softwares, error corrections, and applications

作者： Xiong, Ai-Sheng; Peng, Ri-He; Zhuang, Jing; Gao, Feng; Yao, Quan-Hong; Cheng, Zong-Ming; Li, Yi; SCI

机构： Shanghai Acad Agr Sci, Biotechnol Res Inst, Shanghai 201106, Peoples R China; Univ Tennessee, Dept Plant Sci, Knoxville, TN USA; Univ Connecticut, Dept Plant Sci, Storrs, CT 06269 USA;

来源：FEMS MICROBIOLOGY REVIEWS

关键词： chemical gene synthesis PCR primer design error correction application biotechnology HIGH-LEVEL EXPRESSION SITE-DIRECTED MUTAGENESIS OVERLAP EXTENSION PCR HETEROLOGOUS PROTEIN-PRODUCTION HEPATITIS-B-VIRUS FLANKING HOMOLOGY REGIONS POLYMERASE-CHAIN-REACTION IN-VITRO RECOMBINATION SYNONYMOUS CODON USAGE TRANSGENIC RICE PLANTS