A simple, rapid, high-fidelity and cost-effective PCR-based two-step DNA synthesis method for long gene sequences

Xiong, AS; Yao, QH; Peng, RH; Li, X; Fan, HQ; Cheng, ZM; Li, Y; SCI

机构： Shanghai Acad Agr Sci, Agrobiotechnol Res Ctr, Shanghai 201106, Peoples R China; Shanghai Acad Agr Sci, Agrobiotechnol Res Ctr, Shanghai 201106, Peoples R China; Univ Tennessee, Dept Plant Sci, Knoxville, TN 37996 USA; Univ Connecticut, Dept Plant Sci, Storrs, CT 06269 USA;

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Validation of a rice specific gene, sucrose phosphate synthase, used as the endogenous reference gene for qualitative and real-time quantitative PCR detection of transgenes

Ding, JY; Jia, JW; Yang, LT; Wen, HB; Zhang, CM; Liu, WX; Zhang, DB; SCI

机构： Shanghai Acad Agr Sci, Agrobiotech Res Ctr, Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China; Shanghai Acad Agr Sci, Agrobiotech Res Ctr, Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China; Nanjing Univ, Dept Biol Sci & Technol, Nanjing 210093, Peoples R China; Shanghai Univ, Sch Life Sci, Shanghai 200436, Peoples R China;

关键词： Oryza sativa rice sucrose phosphate synthase qualitative PCR quantitative PCR endogenous reference gene GMOs detection

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Development of an efficient method for the isolation of factors involved in gene transcription during rice embryo development

Ye, R; Yao, QH; Xu, ZH; Xue, HW; SCI

机构： Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Natl Lab Plant ol Genet, Shanghai 200032, Peoples R China; Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Natl Lab Plant ol Genet, Shanghai 200032, Peoples R China; Max Planck Inst Mol Plant Physiol Plant Mol Physi, Partner Grp, Shanghai 200032, Peoples R China; Shanghai Acad Agr Sci, Shanghai Key Lab Agr Genet & Breeding, Agrobiotechnol Res Ctr, Shanghai 201106, Peoples R China;

关键词： transcription factors activation Oryza sativa seed development

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Tomato stress-responsive factor TSRF1 interacts with ethylene responsive element GCC box and regulates pathogen resistance to Ralstonia solanacearum

Zhang, HB; Zhang, DB; Chen, J; Yang, YD; Huang, ZJ; Huang, DF; Wang, XC; Huang, RF; SCI

机构： Chinese Acad Agr Sci, Natl Plant Gene Res Ctr Beijing, Beijing 100081, Peoples R China; Chinese Acad Agr Sci, Natl Plant Gene Res Ctr Beijing, Beijing 100081, Peoples R China; Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China; China Agr Univ, Coll Biol Sci, Natl Lab Plant Physiol & Biochem, Beijing 100094, Peoples R China; Shanghai Acad Agr Sci, Biotechnol Res Ctr, Shanghai 201106, Peoples R China; Chinese Acad Agr Sci, Vegetable & Flower Inst, Beijing 100081, Peoples R China;

关键词： ethylene signaling GCC box PR gene expression resistance to Ralstonia solanacearum transient expression TSRF1

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Engineering higher yield and herbicide resistance in rice by Agrobacterium-mediated multiple gene transformation

Cao, MX; Huang, JQ; Wei, ZM; Yao, QH; Wan, CZ; Lu, JA; SCI

机构： Chinese Acad Sci, Shanghai Inst Biol Sci, Natl Lab Plant Mol Genet, Shanghai 200032, Peoples R China; Chinese Acad Sci, Shanghai Inst Biol Sci, Natl Lab Plant Mol Genet, Shanghai 200032, Peoples R China; Shanghai Acad Agr Sci, Agrobiotech Res Ctr, Shanghai 201106, Peoples R China; Shanghai Acad Agr Sci, Crop Breeding & Cultivat Res Inst, Shanghai 201106, Peoples R China;

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Production of FaeG, the major subunit of K88 fimbriae, in transgenic tobacco plants and its immunogenicity in mice

Huang, YH; Liang, WQ; Pan, AH; Zhou, Z; Huang, C; Chen, JX; Zhang, DB; SCI

机构： Shanghai Acad Agr Sci, Agro Biotech Res Ctr, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China; Shanghai Acad Agr Sci, Agro Biotech Res Ctr, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China; Nanjing Univ, Sch Life Sci, Dept Biol Sci & Technol, Nanjing, Peoples R China; Shanghai Acad Agr Sci, Agro Biotech Res Ctr, Inst Anim & Vet Sci, Shanghai 201106, Peoples R China;

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Multiplex polymerase chain reaction method for detection of bovine materials in foodstuffs

Gao, HW; Zhang, DB; Pan, AH; Liang, WQ; Liang, CZ; SCI

机构： Shanghai Acad Agr Sci, Shanghai Key Lab Agr Genet & Breeding, Agrobiotech Res Ctr, Shanghai, Peoples R China; Shanghai Acad Agr Sci, Shanghai Key Lab Agr Genet & Breeding, Agrobiotech Res Ctr, Shanghai, Peoples R China; Qingdao Engry Exit Inspect & Quarantine Bur, Dept Food Adm, Qingdao, Peoples R China;

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Influence of signal peptide sequences on the expression of heterogeneous proteins in Pichia pastoris

Xiong, AS; Peng, RH; Li, X; Fan, HQ; Yao, QH; Guo, MJ; Zhang, SL; SCI

机构： Shanghai Acad Agr Sci, Agrobiotechnol Res Ctr, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China; Shanghai Acad Agr Sci, Agrobiotechnol Res Ctr, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China; E China Univ Sci & Technol, Dept Bioengn, Shanghai 200237, Peoples R China;

关键词： Pichia pastoris synthetic signal sequence expression vector high expression

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A novel real-time quantitative PCR method using attached universal template probe

Zhang, YL; Zhang, DB; Li, WQ; Chen, JQ; Peng, YF; Cao, W; SCI

机构： Shanghai Acad Agr Sci, Agrobiotech Res Ctr, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China; Shanghai Acad Agr Sci, Agrobiotech Res Ctr, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China; Nanjing Univ, Dept Biol Sci & Technol, Nanjing 210093, Peoples R China; GenoMultix Co Ltd, Dept Technol, Shanghai 200233, Peoples R China; Chinese Acad Agr Sci, Inst Crop Protect, Beijing 100094, Peoples R China;

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Ubiquitin-conjugating enzyme (E-2) confers rice UV protection through phenylalanine ammonia-lyase gene promoter unit

Peng, RH; Yao, QH; Xiong, AS; Fan, HQ; Li, X; Peng, YL; SCI

机构： Shanghai Acad Agr Sci, Shanghai Key Lab Agr Genet & Breeding, AgroBiotechnol Res Ctr, Shanghai 201106, Peoples R China; Shanghai Acad Agr Sci, Shanghai Key Lab Agr Genet & Breeding, AgroBiotechnol Res Ctr, Shanghai 201106, Peoples R China; China Agr Univ, Dept Plant Pathol, Beijing 100094, Peoples R China;

关键词： rice ubiquitin-conjugating enzyme UV protection cis-element yeast one-hybrid system

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