Enhancing the Thermal Resistance of a Novel Acidobacteria-Derived Phytase by Engineering of Disulfide Bridges

文献类型： 外文期刊

第一作者： Miao, Renyun

作者： Miao, Renyun;Liu, Tianhai;Cao, Xuelian;Wu, Xiang;Xie, Liyuan;Huang, Zhongqian;Peng, Weihong;Gan, Bingcheng;Tan, Hao;Miao, Renyun;Liu, Tianhai;Cao, Xuelian;Wu, Xiang;Xie, Liyuan;Huang, Zhongqian;Peng, Weihong;Gan, Bingcheng

作者机构：

关键词： Phytase; acidophilic; acidostable; site-directed mutagenesis; disulfide bridge; thermostable

期刊名称：JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：2.351； 五年影响因子：2.65 ）

ISSN： 1017-7825

年卷期： 2016 年 26 卷 10 期

页码：

收录情况： SCI

摘要： A novel phytase of Acidobacteria was identified from a soil metagenome, cloned, overexpressed, and purified. It has low sequence similarity (<44%) to all the known phytases. At the optimum pH (2.5), the phytase shows an activity level of 1,792 mu mol/min/mg at physiological temperature (37 degrees C) and could retain 92% residual activity after 30 min, indicating the phytase is acidophilic and acidostable. However the phytase shows poor stability at high temperatures. To improve its thermal resistance, the enzyme was redesigned using Disulfide by Design 2.0, introducing four additional disulfide bridges. The half-life time of the engineered phytase at 60 degrees C and 80 degrees C, respectively, is 3.0x and 2.8x longer than the wild-type, and its activity and acidostability are not significantly affected.

分类号： Q93

  • 相关文献
作者其他论文 更多>>

微信小程序