Enhancing the Thermal Resistance of a Novel Acidobacteria-Derived Phytase by Engineering of Disulfide Bridges
文献类型： 外文期刊
第一作者： Miao, Renyun
作者： Miao, Renyun;Liu, Tianhai;Cao, Xuelian;Wu, Xiang;Xie, Liyuan;Huang, Zhongqian;Peng, Weihong;Gan, Bingcheng;Tan, Hao;Miao, Renyun;Liu, Tianhai;Cao, Xuelian;Wu, Xiang;Xie, Liyuan;Huang, Zhongqian;Peng, Weihong;Gan, Bingcheng
作者机构：
关键词： Phytase; acidophilic; acidostable; site-directed mutagenesis; disulfide bridge; thermostable
期刊名称：JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：2.351； 五年影响因子：2.65 ）
ISSN： 1017-7825
年卷期： 2016 年 26 卷 10 期
页码：
收录情况： SCI
摘要： A novel phytase of Acidobacteria was identified from a soil metagenome, cloned, overexpressed, and purified. It has low sequence similarity (<44%) to all the known phytases. At the optimum pH (2.5), the phytase shows an activity level of 1,792 mu mol/min/mg at physiological temperature (37 degrees C) and could retain 92% residual activity after 30 min, indicating the phytase is acidophilic and acidostable. However the phytase shows poor stability at high temperatures. To improve its thermal resistance, the enzyme was redesigned using Disulfide by Design 2.0, introducing four additional disulfide bridges. The half-life time of the engineered phytase at 60 degrees C and 80 degrees C, respectively, is 3.0x and 2.8x longer than the wild-type, and its activity and acidostability are not significantly affected.
分类号： Q93
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