Integrated multiple environmental tests and QTL mapping uncover novel candidate genes for seed oil content in upland cotton

文献类型： 外文期刊

第一作者： Dong, Yaxin

作者： Dong, Yaxin;Wu, Jianyong;Song, Jikun;Zhang, Meng;Shahzad, Kashif;Zhang, Xuexian;Guo, Liping;Qi, Tingxiang;Tang, Huini;Qiao, Xiuqin;Xing, Chaozhu;Wu, Jianyong;Shi, Liping

作者机构：

关键词： Upland cotton; RIL population; Oil content; QTL mapping; Fatty acid synthesis

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：5.6； 五年影响因子：5.7 ）

ISSN： 0926-6690

年卷期： 2024 年 220 卷

页码：

收录情况： SCI

摘要： Upland cotton is a prime agricultural product that is regarded as the backbone of the world's economy and people's livelihood. Recently, cottonseed oil has gradually become a promising edible oil target because of its rich unsaturated fatty acids (FAs). However, genetic resources available for improving cottonseed oil content (OC) are currently very scarce. Here, a recombinant inbred line (RIL) population containing 140 families in upland cotton was specifically developed and utilized for multiple environmental tests and linkage mapping of cottonseed OC. A total of 25 QTLs were identified under nine environments, including six stable QTLs that were potentially interlinked with cottonseed OC. Forty-five candidate genes involved in lipid synthesis processes were further obtained in the stable QTL regions. Based on published transcriptome and qRT-PCR validation, GhBCP_A05 and GhPAHX_D01 were screened to explore their functional effects on cottonseed OC and FAs. Through cloning these two gene from parents, only one genotype for GhBCP_A05 was obtained, while two genotypes named as GhPAHX_D01(MA) and GhPAHX_D01(PC) were obtained for GhPAHX_D01. . It was determined that the GhBCP_A05 protein was located in cytomembrane and nuclear membrane, and the GhPAHX_D01 protein was located in chloroplast by subcellular localization experiments. The ectopic expression in yeast and tobacco indicated that GhBCP_A05 decreased OC and partial FAs content, but GhPAHX_D01(PC) increased OC and partial FAs content. Our findings of this study will not only contribute to further understanding the complex regulatory mechanisms of cottonseed oil biosynthesis but also provide new genetic resources for future targeted design of seed oil-related traits in cotton.

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