I267L Is Neither the Virulence- Nor the Replication-Related Gene of African Swine Fever Virus and Its Deletant Is an Ideal Fluorescent-Tagged Virulence Strain

文献类型： 外文期刊

第一作者： Zhang, Yanyan

作者： Zhang, Yanyan;Zhang, Jingyuan;Yue, Huixian;Chen, Teng;Li, Qian;Zhou, Xintao;Qi, Yu;Zhu, Rongnian;Wang, Shuchao;Miao, Faming;Zhang, Shoufeng;Li, Nan;Mi, Lijuan;Yang, Jinmei;Han, Xun;Wang, Lidong;Hu, Rongliang;Ke, Junnan;Yang, Jinjin;Li, Ying

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关键词： African swine fever virus (ASFV); I267L; deletion; virulence; replication

期刊名称：VIRUSES-BASEL （ 影响因子：5.818； 五年影响因子：5.811 ）

ISSN：

年卷期： 2022 年 14 卷 1 期

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收录情况： SCI

摘要： African swine fever virus (ASFV) is the causative agent of African swine fever (ASF) which reaches up to 100% case fatality in domestic pigs and wild boar and causes significant economic losses in the swine industry. Lack of knowledge of the function of ASFV genes is a serious impediment to the development of the safe and effective vaccine. Herein, I267L was identified as a relative conserved gene and an early expressed gene. A recombinant virus (SY18 Delta I267L) with I267L gene deletion was produced by replacing I267L of the virulent ASFV SY18 with enhanced green fluorescent protein (EGFP) cassette. The replication kinetics of SY18 Delta I267L is similar to that of the parental isolate in vitro. Moreover, the doses of 10(2.0) TCID50 (n = 5) and 10(5.0) TCID50 (n = 5) SY18 Delta I267L caused virulent phenotype, severe clinical signs, viremia, high viral load, and mortality in domestic pigs inoculated intramuscularly as the virulent parental virus strain. Therefore, the deletion of I267L does not affect the replication or the virulence of ASFV. Utilizing the fluorescent-tagged virulence deletant can be easy to gain a visual result in related research such as the inactivation effect of some drugs, disinfectants, extracts, etc. on ASFV.

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