Selection and development of representative simple sequence repeat primers and multiplex SSR sets for high throughput automated genotyping in maize

文献类型： 外文期刊

第一作者： Wang FengGe

作者： Wang FengGe;Zhao JiuRan;Dai JingRui;Yi HongMei;Kuang Meng;Sun YanMei;Yu XinYan;Guo JingLun;Wang Lu

作者机构：

关键词： maize;core primer;SSR;multiplex PCR;capillary electrophoresis;fluorescent-labelled

期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： In the current study, 1900 maize simple sequence repeat (SSR) primers published in MaizeGDB were screened utilizing reference literature, 15 representative Chinese maize inbred lines and 15 Chinese maize hybrids from national regional testing. In total, 500 highly polymorphic primers were identified and used to construct a genetic map. 100 evenly distributed primers, 10 primers per chromosome, were further selected as a set of universal SSR core primers, recommended as preferred primers for general studies. These core primers were then redesigned and used to construct a high throughput multiplex PCR system based on a five-color fluorescence capillary detection system. We report here that two sets of ten-plex PCR combinations have been constructed, each consisting of 10 primers, with one primer per chromosome.

分类号： N1

  • 相关文献

[1]Efficient multiplex simple sequence repeat genotyping of the oomycete plant pathogen Phytophthora infestans. Li, Ying,Cooke, David E. L.,Li, Ying,Jacobsen, Evert,Li, Ying,van der Lee, Theo. 2013

[2]QTL mapping of resistance to sheath blight in maize (Zea Mays L.). Yang, H,Yang, JP,Rong, TZ,Tan, J,Qiu, ZG.

[3]Development and characterization of a core set of SSR markers for fingerprinting analysis of Chinese maize varieties. Wang, Feng-Ge,Tian, Hong-Li,Zhao, Jiu-Ran,Yi, Hong-Mei,Wang, Lu,Song, Wei. 2011

[4]Genetic diversity and relationships among Chinese maize inbred lines revealed by SSR markers. Li, Y,Du, J,Wang, T,Shi, Y,Song, Y,Jia, J. 2002

[5]QTL mapping of coleorhiza length in maize (Zea mays L.) under two germination environmental conditions. Jiang, Xuwen,Tian, Baohua,Zhang, Weimin,Wang, Guoying,Wang, Jianhua,Jiang, Xuwen,Tian, Baohua,Zhang, Weimin,Wang, Guoying,Wang, Jianhua,Wang, Guoying. 2011

[6]Genetic analysis and QTL mapping of maize yield and associate agronomic traits under semi-arid land condition. Guo, Jiufeng,Zhang, Jinpeng,Wang, Guoying,Wang, Guoying,Guo, Jiufeng,Su, Guoqin. 2008

[7]Genetic relationships among CIMMYT subtropical QPM and Chinese maize inbred lines based on SSRS. Li, M. S.,Li, X. H.,Salvi, S.,Tuberosa, R.,Yuan, L. X.,Rotono, F.,Bai, L.,Zhang, S. H.. 2006

[8]Correlation Analysis of Yield and Photosynthetic Traits with Simple Repeat Sequence (SSR) Markers in Maize. Li, Weizhong,Zhao, Dongxu,Wei, Shi,Li, Jing,Li, Weizhong,Wang, Maoqing,Hu, Guohua,Liang, Chunbo. 2017

[9]Homologous analysis of SSR-ESTs and transferability of wheat SSR-EST markers across barley, rice and maize. Tang, Jifeng,Gao, Lifeng,Cao, Yongsheng,Jia, Jizeng. 2006

[10]Analysis of methylation-sensitive amplified polymorphism in different cotton accessions under salt stress based on capillary electrophoresis. Baohua Wang,Rong Fu,Mi Zhang,Zhenqian Ding,Lei Chang,Xinyu Zhu,Yafeng Wang,Baoxiang Fan,Wuwei Ye,Youlu Yuan.

[11]Modified capillary electrophoresis based measurement of the binding between DNA aptamers and an unknown concentration target. Zhang, Yue-Wei,Yan, Hai-Yu,Fu, Ping,Jiang, Fei,Zhang, Yao,Wu, Wen-Xue,Li, Jin-Xiang.

[12]On-line pre-concentration and UV determination of DNA fragments by dynamic coating capillary electrophoresis and its application to detection of genetically modified oilseed rape based on PCR. Chen, Hong,Wu, Yu-Hua,Song, Dan-Yang,Zhang, Wen,Dong, Xu-Yan,Li, Pei-Wu,Lu, Chang-Ming.

[13]A temperature-tolerant multiplex elements and genes screening system for genetically modified organisms based on dual priming oligonucleotide primers and capillary electrophoresis. Fu, Wei,Wang, Chenguang,Zhu, Pengyu,Zhu, Shuifang,Wei, Shuang,Wang, Chenguang,Du, Zhixin,Wu, Xiyang,Wu, Gang.

[14]Capillary electrophoresis and open tubular capillary electrochromatography using a magnesia-zirconia coated capillary. Xie, MJ,Feng, YQ,Da, SL,Meng, DY,Ren, LW.

[15]A highly efficient capillary electrophoresis-based method for size determination of water-soluble CdSe/ZnS core-shell quantum dots. Li, Yong-Qiang,Wang, Hai-Qiao,Wang, Jian-Hao,Guan, Li-Yun,Liu, Bi-Feng,Zhao, Yuan-Di,Chen, Hong. 2009

[16]Effect of exogenous NH4+-N supply on distribution of ureide content in various tissues of alfalfa plants, Medicago sativa. Cheng, XG,Nomura, M,Sato, T,Fujikake, H,Ohyama, T,Tajima, S. 1999

[17]Self-assembly of cellulose nanoparticles as electrolyte additive for capillary electrophoresis separation. Huang, Dihui,Yang, Qin,Jin, Shanxia,Zhou, Ping,Deng, Qianchun.

[18]Rapid diagnosis of goose viral infections by multiplex PCR. Chen, Zongyan,Li, Chuanfeng,Li, Guoxin,Yu, Hai,Jiang, Yifeng,Yan, Liping,Meng, Chunchun,Zhou, Yanjun,Tong, Guangzhi,Liu, Guangqing.

[19]Optimization of a multiplex PCR assay for detecting transgenic soybean components in feed products. Wang, Xiumin,Teng, Da,Yang, Yalin,Guan, Qingfeng,Wang, Jianhua,Tian, Fang,Wang, Xiumin,Teng, Da,Yang, Yalin,Guan, Qingfeng,Wang, Jianhua,Tian, Fang,Ao, Changjin.

[20]Rapid identification of deer products by multiplex PCR assay. Zha, Dai-Ming,Xing, Xiu-Mei,Yang, Fu-He,Zha, Dai-Ming,Zha, Dai-Ming,Xing, Xiu-Mei,Yang, Fu-He,Zha, Dai-Ming,Xing, Xiu-Mei,Yang, Fu-He.

作者其他论文 更多>>

微信小程序