Comparison of heterologous prime-boost immunization strategies with DNA and recombinant vaccinia virus co-expressing GP3 and GP5 of European type porcine reproductive and respiratory syndrome virus in pigs

文献类型： 外文期刊

第一作者： Ren, Jingqiang

作者： Ren, Jingqiang;Ren, Jingqiang;Zhang, Hewei;Lid, Jiachen;Zhai, Chongkai;Mao, Fuchao;Liu, Zhongyu;Zhang, Hewei;Ren, Jingqiang;Lid, Jiachen;Zhai, Chongkai;Mao, Fuchao;Yang, Shaozhe;Zhang, Qingwei;Liu, Zhongyu;Fu, Xiuhong;Ren, Jingqiang;Ren, Jingqiang;Ren, Jingqiang

作者机构：

关键词： Porcine reproductive and respiratory syndrome; virus; Heterologous prime-boost; DNA vaccine; Vaccinia virus vaccine; Adjuvant

期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.8； 五年影响因子：4.0 ）

ISSN： 0882-4010

年卷期： 2023 年 183 卷

页码：

收录情况： SCI

摘要： Vaccination is principally used to control and treat porcine reproductive and respiratory syndrome virus (PRRSV) infection. This study investigated immunogenicity and protective efficacy of heterologous prime-boost regimens in pigs, including recombinant DNA and vaccinia virus vectors coexpressing PRRSV European genotype (EU) isolate GP3 and GP5: group A, pVAX1-EU-GP3-GP5 prime and rddVTT-EU-GP3-GP5 boost; group B, rddVTT-EU-GP3-GP5 prime and pVAX1-EU-GP3-GP5 boost; group C, empty vector pVAX1; group D, E3L gene-deleted vaccinia virus E3L-VTT. Vaccine efficacy was tested in an EU-type PRRSV (Lelystad virus strain) challenge pig model based on evaluating PRRSV-specific antibody responses, neutralizing antibodies, cytokines, T lymphocyte proliferation, CD4+ and CD8+ T lymphocytes, clinical symptoms, viremia and tissue virus loads. Plasmid DNA was delivered as chitosan-DNA nanoparticles, and Quil A (Quillaja) was used to increase vaccine efficiency. All piglets were boosted 21 days post the initial inoculation (dpi) and then challenged 14 days later. At 14, 21, 28 and 35 dpi, groups A and B developed significantly higher PRRSV-specific antibody responses compared with control groups C and D. Two weeks after the boost, significant differences in neutralizing anti-body and IFN-gamma levels were observed between groups A, C, D and B. At 49 dpi, groups A and B had markedly increased peripheral blood CD3+CD4+ T cell levels. Following virus challenge, group A showed viremia, but organ virus loads were lower than those in other groups. Thus, a heterologous prime-boost vaccine regimen (rddVTT-EU-GP3-GP5 prime, pVAX1-EU-GP3-GP5 boost) can improve humoral-and cell-mediated immune responses to provide resistance to EU-type PRRSV infection in vivo.

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