Genome-wide analysis of differentially expressed genes and the modulation of PEDV infection in Vero E6 cells

文献类型： 外文期刊

第一作者： Zhang, Hewei

作者： Zhang, Hewei;Wang, Jianke;Sun, Yaru;Cheng, Shipeng;Wu, Hua;Liu, Qinfang;Su, Weiwei;Chen, Zhenhai;Zhang, Junfeng;Shang, Ke;Zhang, Junfeng;Shang, Ke

作者机构：

关键词： PEDV; RNA-Seq; mTOR signaling pathway; Antiviral

期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.738； 五年影响因子：3.663 ）

ISSN： 0882-4010

年卷期： 2018 年 117 卷

页码：

收录情况： SCI

摘要： PEDV remains one of the most important swine diseases that infects pigs of all ages. It causes devastating viral enteric disease in piglets with a high mortality rate, leading to significant threats and huge economic loss to the pork industry. In this study, a transcriptomic shotgun sequencing (RNA-Seq) procedure was used to study gene responses against PEDV infection. Genome-wide analysis of differentially expressed genes (DEGs) was performed in Vero E6 cells post-PEDV infection. mTOR signaling pathway activator-MHY1485, and inhibitor-PP242 were used to study the antiviral function. Results revealed that the IRF3 was significantly up-regulated post-PEDV infection. Although most of the IFN-regulatory and -related genes evaluated in this study were either down regulated or remained unchanged, IL11 behaved significantly up-regulated, with the peak at 16 hpi. Nearly 90% of PEDV infections were suppressed in the PP242 pretreated cells whereas the reverse effect was observed in the MYH1485 pretreated cells. Results indicated that the mTOR signaling pathway played a vital role in the PEDV antiviral regulation in the Vero E6 cells. Future studies will contribute to better understand the cellular antiviral mechanism against PEDV.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序