First biochemical characterization of a novel ribonuclease from wild mushroom Amanita hemibapha
文献类型： 外文期刊
第一作者： Ma, Duanzheng
作者： Ma, Duanzheng;Wang, Hexiang;Sekete, Malota;Ma, Duanzheng;Wang, Hexiang;Wang, Bo;Ng, Tzibun
作者机构：
关键词： Ribonuclease; Mushroom; Purification
期刊名称：SPRINGERPLUS （ 影响因子：1.13； 五年影响因子：1.29 ）
ISSN： 2193-1801
年卷期： 2012 年 1 卷
页码：
收录情况： SCI
摘要： A 45-kDa ribonuclease (RNase) was purified from dried fruiting bodies of the wild mushroom Amanita hemibapha. It was adsorbed on DEAE-cellulose, S-sepharose, and finally purified on Superdex 75. The RNase exhibited maximal RNase activity at pH 5 and in a temperature range between 60-70 degrees C. It demonstrated no ribonucleolytic activity toward four polyhomoribonucleotides. The amino acid sequence analysis (GDDETFWEHEWAK) showed this RNase was a ribonuclease T2-like RNase. It exhibited strong inhibitory activity against HIV-1 reverse transcriptase (HIV-1 RT) with an IC50 of 17 mu M.
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