C/EBP alpha Regulates PxTreh1 and PxTreh2 Trehalase-Related Bt Resistance in Plutella xylostella (L.)

文献类型： 外文期刊

第一作者： Liu, Jia

作者： Liu, Jia;Zeng, Aiping;Liu, Jia;Liu, Zheming;Ma, Haihao;Zhang, Chengjia;Zheng, Wei;Man, Yilong;Zhu, Hang;Zhou, Yong;Chen, Xi;Zhou, Xiaomao;Yu, Yaying

作者机构：

关键词： P. xylostella; PxTreh1; PxTreh2; C/EBP alpha; expression regulation

期刊名称：INSECTS （ 影响因子：3.139； 五年影响因子：3.285 ）

ISSN：

年卷期： 2022 年 13 卷 4 期

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收录情况： SCI

摘要： Simple Summary The diamondback moth (Plutella xylostella) is a major agricultural pest of cruciferous vegetables and crops worldwide, causing economic losses of up to USD 5 billion annually. The long-term use of insecticides leads to the rapid evolution of resistance in insects, which greatly increases the difficulty of controlling pests. Trehalase regulates energy metabolism in insects by converting trehalose into two glucose molecules. The existence of trehalase is critical for insect flight and larval stress resistance. However, whether trehalase participates in the development of pesticide resistance remains unclear. In this study, we found that the activity of trehalase and the levels of gene expression in Bt-resistant and field populations of P. xylostella were significantly higher than they were in the susceptible strains. By analyzing the promoter sequences of PxTreh1 and PxTreh2, we confirmed the interaction between C/EBP alpha and the PxTreh2 promoter. The findings of this study suggest that C/EBP alpha mediates the adaptability of P. xylostella to adverse environmental stressors by regulating the expression of trehalase. Trehalase regulates energy metabolism in insects by converting trehalose into two glucose molecules. High amounts of trehalase are critical for insect flight and larval stress resistance. However, whether trehalase participates in the development of pesticide resistance remains unclear. In this study, we explored this phenomenon and the mechanism that underlies the regulation of Trehalase transcription. We found that overexpression of PxTreh1 and PxTreh2 induced Bacillus thuringiensis (Bt) resistance in Plutella xylostella. The promoter sequences of PxTreh1 and PxTreh2 were also cloned and identified. The dual-luciferase reporter system and RNA interference technology revealed that the expression of PxTreh1 and PxTreh2 genes is possibly regulated by the CCAAT enhancer-binding protein (C/EBP alpha). A yeast one-hybrid experiment confirmed the interaction between C/EBP alpha and the PxTreh2 promoter. The findings of this study suggest that C/EBP alpha mediates the adaptability of P. xylostella to adverse environmental stressors by regulating the expression of trehalase.

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