Cry51Aa Proteins Are Active against Apolygus lucorum and Show a Mechanism Similar to Pore Formation Model

文献类型： 外文期刊

第一作者： Soe, Ei Thinzar

作者： Soe, Ei Thinzar;Naing, Zaw Lin;Zhang, Caihong;Niu, Lin Lin;Tang, Jinrong;Yu, Siqi;Ding, Zhongwei;Liang, Gemei

作者机构：

关键词： Apolygus lucorum; Bacillus thuriengiensis; Cry51Aa; toxicity; mode of action

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）

ISSN： 0021-8561

年卷期： 2023 年 71 卷 5 期

页码：

收录情况： SCI

摘要： Reduced insecticide spray in crop fields due to the widespread adoption of Bacillus thuringiensis (Bt) crops has favored the population increases of mirid bugs. Cry51Aa proteins are new types of Bt proteins that belong to aerolysin-like beta pore-forming proteins with insecticidal activity against hemipteran and coleopteran pests. Here, we studied the activity of Bt Cry51Aa1 and Cry51Aa2 against Apolygus lucorum, an emerging pest in cotton, and their mechanism of action. Cry51Aa1 exhibited almost 5-fold higher toxicity than Cry51Aa2 with LC50 of 11.87 and 61.34 mu g/mL, respectively. Protoxins could be activated both in vitro, by trypsin and midgut contents, and in vivo, by A. lucorum midgut. Both Cry51Aa protoxins were processed in two steps, producing pre-activated (similar to 30 kDa) and final activated (similar to 25-28 kDa) proteins. Cry51Aa proteins bound to a 25 kDa midgut protein, and Cry51Aa2 showed 2 times higher binding affinity than Cry51Aa1. Incubating Cry51Aa proteins with midgut homogenate resulted in toxin oligomers of 150-200 kDa. Our findings provide a theoretical basis for using Cry51Aa proteins to control A. lucorum and a better understanding of their mode of action.

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