Characterisation and immunostimulatory activity of an alpha-(1 -> 6)-D-glucan from the cultured Armillariella tabescens mycelia

文献类型： 外文期刊

第一作者： Yu, Mengyao

作者： Yu, Mengyao;Yang, Zhirong;Zheng, Linyong;Xu, Xiaoyan;Yu, Mengyao;Zheng, Linyong

作者机构：

关键词： Armillariella tabescens; polysaccharide; macrophage; nitric oxide; cytokines

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN： 0308-8146

年卷期： 2008 年 111 卷 2 期

页码：

收录情况： SCI

摘要： IPS-B2, an intracellular polysaccharide with anti-tumor effect, was isolated from cultured Armillariella tabescens mycelia by hot water extraction, anion-exchange and gel chromatography. Based on the results of paper chromatography (PC), gas chromatography (GC), infra-red (IR) spectroscopy and C-13 NMR, IPS-B2 was characterized as an alpha-(1 -> 6)-D-glucan with a molecular weight of 49.5 kDa. The effects of IPS-B2 on murine peritoneal macrophages were further investigated. The results demonstrated that IPS-B2 induced nitric oxide (NO) and cytokines (TNF-alpha, IL-1 beta and IL-6) production in macrophages. The outcomes of real-time reverse transcription-polymerase chain reaction (RT-PCR) proved that the transcribing level of inducible NO synthase (iNOS), TNF-alpha, IL-1 beta and IL-6 mRNA in the peritoneal macrophages have been augmented by IPS-B2. These data suggest that the anti-tumor activity of the polysaccharide from A. tabescens may due to activation of macrophage. (C) 2008 Elsevier Ltd. All rights reserved.

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