Isolation and characterization of a reovirus causing spleen necrosis in Pekin ducklings
文献类型: 外文期刊
第一作者: Liu, Qinfang
作者: Liu, Qinfang;Zhang, Guozhong;Ren, Gaixian;Chen, Liben;Gao, Jian;Zhang, Dabing;Han, Bo;Su, Wenliang;Zhao, Jixun;Su, Jingliang;Hu, Xueying;Huang, Yu
作者机构:
关键词: Pekin duckling;Spleen necrosis;Reovirus;Chicken
期刊名称:VETERINARY MICROBIOLOGY ( 影响因子:3.293; 五年影响因子:3.599 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: High rates of mortality for Pekin ducklings have been recorded in several duck farms in China since 2006. Dead ducklings were characterized by spleen necrosis, suggesting microbial infection as a cause of disease. Laboratory investigations led to the isolation of a virus strain from the spleen tissues of dead ducklings, designated DRV-HC. Subsequent experimental infections with DRV-HC resulted in marked spleen necrosis in the ducklings similar to those observed in the natural outbreaks. Electron microscopy of the cultured DRV-HC revealed viral particles that were non-enveloped and icosahedral with a mean diameter of approximately 72 nm. Agar gel precipitating tests showed that the isolate shared a common group-specific antigen with chicken reovirus S1133. DNA sequencing revealed that this isolate was closely related to Muscovy duck reoviruses. Experimental infection with DRV-HC resulted in death of young chicks with necrotic foci in the liver and spleen. To the best of our knowledge, this is the first report of the isolation of a duck reovirus with high virulence in Pekin ducklings and SPF chickens.
分类号: S8
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