Paracoccus broussonetiae subsp. drimophilus subsp. nov., a Novel Subspecies Salt-Tolerant Endophytic Bacterium from Maize Root in Hunan

文献类型： 外文期刊

第一作者： Zhou, Chi

作者： Zhou, Chi;Zhang, Qingzhuang;Li, Xin;Li, Xue;Su, Lei;Zhou, Chi;Zhang, Qingzhuang;Li, Xin;Li, Ming

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关键词： endophytic bacterium; polyphasic taxonomy; salt-tolerant bacterium

期刊名称：LIFE-BASEL （ 影响因子：3.4； 五年影响因子：3.4 ）

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年卷期： 2025 年 15 卷 3 期

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收录情况： SCI

摘要： In an investigation exploring endophytic microbiota from agricultural crops, an aerobic, non-motile, Gram-negative, coccobacillus-shaped bacterial isolate, designated as strain NGMCC 1.201697T, was isolated from maize roots in Hunan Province, China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NGMCC 1.201697T belonged to the genus Paracoccus, showing the highest sequence similarity to Paracoccus broussonetiae CPCC 101403T (99.86%). The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) were 98.57% and 87.90% between the novel isolate and its closest phylogenetic relative. However, phenotypic characterization further differentiated the isolate from P. broussonetiae CPCC 101403T. The isolate showed enhanced environmental tolerance adaptability (growth in 0-8% NaCl and 4-37 degrees C), unique enzymatic activities (esterase C4, beta-glucosidase, L-proline arylamidase, and beta-galactosidase), and expanded metabolic capabilities (D-mannitol, D-cellobiose, saccharose, and so on). The major polar lipids consisted of diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylglycerol (PG), two unidentified glycolipids (GLs) and four unidentified phospholipids (PLs). The predominant respiratory quinone was ubiquinone-10, and the major fatty acid was summed feature 8 (C18:1 omega 7c, 69.42%). The DNA G + C content was 64.49 mol%. Based on results of these analyses, strain NGMCC 1.201697T represents a novel subspecies of Paracoccus broussonetiae, for which the name Paracoccus broussonetiae subsp. drimophilus subsp. nov. is proposed. The type-strain is NGMCC 1.201697T (=CGMCC 1.61958T =JCM 37104T).

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