Krüppel-homolog 1 is key in development and parthenogenetic reproduction in pea aphids

文献类型： 外文期刊

第一作者： Song, Zichao

作者： Song, Zichao;Yan, Zuqing;Zhao, Yanfen;Zhang, Aiyu;Liu, Fanghua;Sun, Jianghua;Kang, Zhiwei;Gao, Yuqing;Xu, Yongyu;Kang, Zhiwei;Tian, Honggang;Tian, Honggang;Li, Sheng;Wang, Su;Smagghe, Guy;Liu, Tong-Xian;Smagghe, Guy;Smagghe, Guy

作者机构：

关键词： Acyrthosiphon pisum; metamorphosis; cuticle proteins; fecundity; regulation mechanism

期刊名称：ENTOMOLOGIA GENERALIS （ 影响因子：4.6； 五年影响因子：4.4 ）

ISSN： 0171-8177

年卷期： 2025 年 45 卷 1 期

页码：

收录情况： SCI

摘要： Kr & uuml;ppel-homolog 1 (Kr-h1) is a key juvenile hormone (JH)-induced transcription factor and plays an important role in insect development and reproduction. However, the molecular function of Kr-h1 in aphids is still unknown. In this study, we used the parthenogenetic pea aphid, Acyrthosiphon pisum, to investigate the presence and role of ApKr-h1 in postembryonic development and reproduction. The tissue distribution and developmental profiles demonstrated that ApKr-h1 was highly expressed in 4th-instar nymph and adults and embryo. Knockdown of ApKr-h1 in 4th-instar nymph resulted in a precocious molting and 79% of aphids were arrested in their molting process. The rest aphids that were able to successfully develop into adults failed to sclerotize normally and died rapidly without producing offspring. Hence, RNA-seq and functional assays revealed that ApKr-h1 can directly regulate the expression of a cuticle protein ApCP53 by binding to its promoter, potentially explaining the failure of molting. Moreover, injection of adults with dsApKr-h1 significantly decreased the fecundity of A. pisum. Our findings not only enrich our knowledge on the function of the Kr-h1 and JH in the development with molting process and reproduction of A. pisum, but may also provide potential targets for RNAi-based management of aphids employing JH-related genes.

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