Development and evaluation of the utility of GenoBaits Peanut 40K for a peanut MAGIC population

文献类型： 外文期刊

第一作者： Sun, Ziqi

作者： Sun, Ziqi;Zheng, Zheng;Qi, Feiyan;Wang, Juan;Wang, Mengmeng;Zhao, Ruifang;Liu, Hua;Xu, Jing;Qin, Li;Dong, Wenzhao;Huang, Bingyan;Han, Suoyi;Zhang, Xinyou

作者机构：

关键词： GenoBaits Peanut 40K; GBTS liquid chip; MAGIC population; Pod size; Peanut

期刊名称：MOLECULAR BREEDING （ 影响因子：3.1； 五年影响因子：3.1 ）

ISSN： 1380-3743

年卷期： 2023 年 43 卷 10 期

页码：

收录情况： SCI

摘要： Population and genotype data are essential for genetic mapping. The multi-parent advanced generation intercross (MAGIC) population is a permanent mapping population used for precisely mapping quantitative trait loci. Moreover, genotyping-by-target sequencing (GBTS) is a robust high-throughput genotyping technology characterized by its low cost, flexibility, and limited requirements for information management and support. In this study, an 8-way MAGIC population was constructed using eight elite founder lines. In addition, GenoBaits Peanut 40K was developed and utilized for the constructed MAGIC population. A subset (297 lines) of the MAGIC population at the S2 stage was genotyped using GenoBaits Peanut 40K. Furthermore, these lines and the eight parents were analyzed in terms of pod length, width, area, and perimeter. A total of 27 single nucleotide polymorphisms (SNPs) were revealed to be significantly associated with peanut pod size-related traits according to a genome-wide association study. The GenoBaits Peanut 40K provided herein and the constructed MAGIC population will be applicable for future research to identify the key genes responsible for important peanut traits.

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