Isolation and characterization of microsatellite DNA loci from the southern flounder, Paralichthys lethostigma

文献类型： 外文期刊

第一作者： Shao, C. W.

作者： Shao, C. W.;Liao, X.;Chen, S. L.;Shao, C. W.

作者机构：

关键词： enriched genomic library;microsatellites;Paralichthys lethostigma

期刊名称：MOLECULAR ECOLOGY RESOURCES （ 影响因子：7.09； 五年影响因子：8.745 ）

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收录情况： SCI

摘要： Paucity of polymorphic molecular markers in southern flounder (Paralichthys lethostigma) has been a major limitation in genetic improvement of this important economic fish. Hence, we constructed a repeat-enriched genomic library from P. lethostigma. A total of 39 new microsatellites were identified, for which 33 primer pairs were designed. After validating and scoring, 10 of these loci were polymorphic in a test population with the range of alleles from two to nine per locus. The observed and expected heterozygosities ranged from 0.2500 to 0.9000 and from 0.4469 to 0.8514, respectively. These polymorphic microsatellites will be useful for genetic diversity analysis and linkage map construction for P. lethostigma.

分类号： Q7

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