Control of axillary bud growth in tobacco through toxin gene expression system

文献类型： 外文期刊

第一作者： Lv, Jing

作者： Lv, Jing;Chen, Ya-Qiong;Ding, An-Ming;Gao, Xiao-Ming;Dai, Chang-Bo;Sun, Yu-He;Lv, Jing;Chen, Ya-Qiong;Ding, An-Ming;Gao, Xiao-Ming;Dai, Chang-Bo;Sun, Yu-He;Lv, Jing;Lei, Bo;Yu, Jing;Chen, Ya-Qiong

作者机构：

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.38； 五年影响因子：5.134 ）

ISSN： 2045-2322

年卷期： 2021 年 11 卷 1 期

页码：

收录情况： SCI

摘要： The control of axillary bud development after removing the terminal buds (topping) of plants is a research hotspot, and the control of gene expression, like switching on and off, allows us to further study biological traits of interest, such as plant branching and fertility. In this study, a toxin gene control system for plants based on dexamethasone (DEX) induction was constructed, and the positive transgenic tobacco exhibited growth retardation in the application area (axillary bud). The expression level of the lethal Diphtheria toxin A (DTA) gene under different DEX concentrations at different application days was analyzed. The highest expression levels appeared at 5 days after the leaf injection of DEX. The DTA transcripts were induced by 5 mu M DEX and peaked in response to 50 mu M DEX at 5 days after leaf injection. Here, a chemical induction system, combined with a toxin gene, were used to successfully control the growth of tobacco axillary buds after topping. The DTA expression system under DEX induction was sensitive and efficient, therefore, can be used to control axillary bud growth and development in tobacco.

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