The unconventional resistance protein PTR recognizes the Magnaporthe oryzae effector AVR-Pita in an allele-specific manner

文献类型: 外文期刊

第一作者: Xiao, Gui

作者: Xiao, Gui;Wu, Jun;Xiao, Gui;Telebanco-Yanoria, Mary Jeanie;Zhou, Bo;Laksanavilat, Nutthalak;Cesari, Stella;Lambou, Karine;Baudin, Mael;Jalilian, Ahmad;Chalvon, Veronique;Meusnier, Isabelle;Fournier, Elisabeth;Tharreau, Didier;Kroj, Thomas;Tharreau, Didier;Baudin, Mael;Jalilian, Ahmad

作者机构:

期刊名称:NATURE PLANTS ( 影响因子:18.0; 五年影响因子:18.6 )

ISSN: 2055-026X

年卷期: 2024 年

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收录情况: SCI

摘要: Blast disease caused by the fungus Magnaporthe oryzae is one of the most devastating rice diseases. Disease resistance genes such as Pi-ta or Pi-ta2 are critical in protecting rice production from blast. Published work reports that Pi-ta codes for a nucleotide-binding and leucine-rich repeat domain protein (NLR) that recognizes the fungal protease-like effector AVR-Pita by direct binding. However, this model was challenged by the recent discovery that Pi-ta2 resistance, which also relies on AVR-Pita detection, is conferred by the unconventional resistance gene Ptr, which codes for a membrane protein with a cytoplasmic armadillo repeat domain. Here, using NLR Pi-ta and Ptr RNAi knockdown and CRISPR/Cas9 knockout mutant rice lines, we found that AVR-Pita recognition relies solely on Ptr and that the NLR Pi-ta has no role in it, indicating that it is not the Pi-ta resistance gene. Different alleles of Ptr confer different recognition specificities. The A allele of Ptr (PtrA) detects all natural sequence variants of the effector and confers Pi-ta2 resistance, while the B allele of Ptr (PtrB) recognizes a restricted set of AVR-Pita alleles and, thereby, confers Pi-ta resistance. Analysis of the natural diversity in AVR-Pita and of mutant and transgenic strains identified one specific polymorphism in the effector sequence that controls escape from PtrB-mediated resistance. Taken together, our work establishes that the M. oryzae effector AVR-Pita is detected in an allele-specific manner by the unconventional rice resistance protein Ptr and that the NLR Pi-ta has no function in Pi-ta resistance and the recognition of AVR-Pita.

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