文献类型： 外文期刊
第一作者： Wu, Han
作者： Wu, Han;Zhang, Kuangye;Zhang, Zhipeng;Wang, Jiaxu;Cong, Ling;Li, Jia;Duan, Youhou;Ke, Fulai;Zhang, Fei;Liu, Zhiqiang;Lu, Feng;Wang, Yanqiu;Li, Zhihua;Zou, Jianqiu;Zhu, Kai;Jia, Pengxiang;Chang, Ming
作者机构：
关键词： Cell -penetrating peptides; Genome editing; DNA -free; RNP; CRISPR
期刊名称：PLANT SCIENCE （ 影响因子：5.363； 五年影响因子：5.454 ）
ISSN： 0168-9452
年卷期： 2022 年 324 卷
页码：
收录情况： SCI
摘要： Genome editing system based on the CRISPR/Cas (clustered regularly interspaced short palindromic repeats) technology is a milestone for biology. However, public concerns regarding genetically modified organisms (GMOs) and recalcitrance in the crop of choice for regeneration have limited its application. Cell-penetrating peptides (CPPs) are derived from protein transduction domains (PTDs) that can take on various cargoes across the plant wall, and membrane of target cells. Selected CPPs show mild cytotoxicity and are a suitable delivery tool for DNA-free genome editing. Moreover, CPPs may also be applied for the transient delivery of morphogenic transcription factors, also known as developmental regulators (DRs), to overcome the bottleneck of the crop of choice regeneration. In this review, we introduce a brief history of cell-penetrating peptides and discuss the practice of CPP-mediated DNA-free transfection and the prospects of this potential delivery tool for improving crop genome editing.
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