Co-ordinate expression of glycine betaine synthesis genes linked by the FMDV 2A region in a single open reading frame in Pichia pastoris

文献类型： 外文期刊

第一作者： Wang, Sanhong

作者： Wang, Sanhong;Yao, Quanhong;Tao, Jianmin;Qiao, Yushan;Zhang, Zhen

作者机构：

关键词： glycine betaine synthesis genes;2A region of foot-and-mouth disease virus;coexpression;stress tolerance;Pichia pastoris;MOUTH-DISEASE VIRUS;ALDEHYDE DEHYDROGENASE;TRANSGENIC PLANTS;ESCHERICHIA-COLI;MULTIPLE GENES;CLEAVAGE;TOLERANCE;CHOLINE;POLYPROTEIN;STRESS

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

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年卷期：

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收录情况： SCI

摘要： The genes encoding the two enzymes choline monooxygenase (CMO) and betaine aldehyde dehydrogenase (BADH) of glycine betaine synthesis in Suaeda salsa were cloned and fused with the 2A region of foot-and-mouth disease virus in a single open reading frame. The fused genes were placed under the control of the alcohol oxidase (AOX1) promoter in pPIC3B and transformed into P. pastoris GS115. The expression of the fused genes in P. pastoris and the ability of recombinant yeasts to tolerate environmental stresses were studied. The results showed that induced with 0.5% methanol for 96 h, the maximal activities of CMO and BADH in the tested recombinant yeasts were 45- and 44-fold higher than those in the control yeast transformed empty vector only, respectively; the content of glycine betaine in the recombinant yeasts was 28- to 35-fold higher than that in the control. The fused genes linked by 2A region of foot-and-mouth disease virus were expressed in P. pastoris successfully and the polyprotein was 'cleaved' to each functional protein. The yeasts transformed the fused genes, which were more resistant to salt, methanol, and high temperature stresses than the control as result of glycine betaine synthesis genes introduced.

分类号： Q93

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