Biochemical profiling of the protein encoded by grass carp reovirus genotype II

文献类型： 外文期刊

第一作者： Liu, Man

作者： Liu, Man;Kong, Xianghui;Liu, Man;Xu, Chen;Zhou, Yong;Xue, Mingyang;Jiang, Nan;Li, Yiqun;Huang, Zhenyu;Meng, Yan;Liu, Wenzhi;Fan, Yuding

作者机构：

期刊名称：ISCIENCE （ 影响因子：4.6； 五年影响因子：5.0 ）

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年卷期： 2024 年 27 卷 8 期

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收录情况： SCI

摘要： In this study, we obtained the whole genome sequence of GCRV-DY197 and investigated the localization, post-translational modifications, and host interactions of the 11 viral proteins encoded by GCRV-DY197 in grass carp ovary (GCO) cells. The whole genome sequence is 24,704 kb and contains 11 segments (S1- S11). Subcellular localization showed that the VP1, VP2, VP3, VP5, VP56, and VP35 proteins were localized in both cytoplasm and nucleus, whereas the NS79, VP4, VP41, VP6, and NS38 proteins were localized in the cytoplasm. The NS79 and NS38 proteins were phosphorylated, and the ubiquitination modification sites were identified in VP41 and NS38. An interaction network containing 9 viral proteins and 140 host proteins was also constructed. These results offer a theoretical basis for an in-depth understanding of the biochemical characteristics and pathogenic mechanism of GCRV-II.

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