DIA-Based Proteomic Analysis Reveals MYOZ2 as a Key Protein Affecting Muscle Growth and Development in Hybrid Sheep

文献类型： 外文期刊

第一作者： Zhang, Dan

作者： Zhang, Dan;Yue, Yaojing;Yuan, Chao;An, Xuejiao;Guo, Tingting;Chen, Bowen;Liu, Jianbin;Lu, Zengkui;Zhang, Dan;Yue, Yaojing;Yuan, Chao;An, Xuejiao;Guo, Tingting;Chen, Bowen;Liu, Jianbin;Lu, Zengkui

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关键词： hybrid sheep; proteomic; MYOZ2; muscle; growth and development

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）

ISSN： 1661-6596

年卷期： 2024 年 25 卷 5 期

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收录情况： SCI

摘要： Hybridization of livestock can be used to improve varieties, and different hybrid combinations produce unique breeding effects. In this study, male Southdown and Suffolk sheep were selected to hybridize with female Hu sheep to explore the effects of male parentage on muscle growth and the development of offspring. Using data-independent acquisition technology, we identified 119, 187, and 26 differentially abundant proteins (DAPs) between Hu x Hu (HH) versus Southdown x Hu (NH), HH versus Suffolk x Hu (SH), and NH versus SH crosses. Two DAPs, MYOZ2 and MYOM3, were common to the three hybrid groups and were mainly enriched in muscle growth and development-related pathways. At the myoblast proliferation stage, MYOZ2 expression decreased cell viability and inhibited proliferation. At the myoblast differentiation stage, MYOZ2 expression promoted myoblast fusion and enhanced the level of cell fusion. These findings provide new insights into the key proteins and metabolic pathways involved in the effect of male parentage on muscle growth and the development of hybrid offspring in sheep.

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