Different populations of A(H1N1)pdm09 viruses in a patient with hemolytic-uremic syndrome

文献类型： 外文期刊

第一作者： Fu, Yuguang

作者： Fu, Yuguang;Fu, Yuguang;Herrler, Georg;Wedde, Marianne;Oh, Djin-Ye;Duwe, Susanne;Biere, Barbara;Reiche, Janine;Schweiger, Brunhilde;Mache, Christin;Wolff, Thorsten;Duerrwald, Ralf;Smola, Sigrun;Pfuhl, Thorsten;Rissland, Jurgen;Zemlin, Michael;Flockerzi, Fidelis A.;Bohle, Rainer M.;Thuermer, Andrea;Duerrwald, Ralf

作者机构：

关键词： Fatal influenza; S263S/F (HA1) and H456H/Y (PB1) mutations

期刊名称：INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY （ 影响因子：4.1； 五年影响因子：3.5 ）

ISSN： 1438-4221

年卷期： 2024 年 314 卷

页码：

收录情况： SCI

摘要： Respiratory viral infections may have different impacts ranging from infection without symptoms to severe disease or even death though the reasons are not well characterized. A patient (age group 5-15 years) displaying symptoms of hemolytic uremic syndrome died one day after hospitalization. qPCR, next generation sequencing, virus isolation, antigenic characterization, resistance analysis was performed and virus replication kinetics in well -differentiated airway cells were determined. Autopsy revealed hemorrhagic pneumonia as major pathological manifestation. Lung samples harbored a large population of A(H1N1)pdm09 viruses with the polymorphism H456H/Y in PB1 polymerase. The H456H/Y viruses replicated much faster to high viral titers than upper respiratory tract viruses in vitro. H456H/Y-infected air -liquid interface cultures of differentiated airway epithelial cells did reflect a more pronounced loss of ciliated cells. A different pattern of virus quasispecies was found in the upper airway samples where substitution S263S/F (HA1) was observed. The data support the notion that viral quasispecies had evolved locally in the lung to support high replicative fitness. This change may have initiated further pathogenic processes leading to rapid dissemination of inflammatory mediators followed by development of hemorrhagic lung lesions and fatal outcome.

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