AlphaFold-guided redesign of a plant pectin methylesterase inhibitor for broad-spectrum disease resistance

文献类型： 外文期刊

第一作者： Xia, Yeqiang

作者： Xia, Yeqiang;Sun, Guangzheng;Xiao, Junhua;He, Xinyi;Jiang, Haibin;Zhang, Zhichao;Zhang, Qi;Li, Kainan;Zhang, Sicong;Shi, Xuechao;Zhao, Yao;Yang, Yuheng;Duan, Kaixuan;Ye, Wenwu;Wang, Yiming;Dong, Suomeng;Wang, Yan;Ma, Zhenchuan;Wang, Yuanchao;Xia, Yeqiang;Sun, Guangzheng;Xiao, Junhua;He, Xinyi;Jiang, Haibin;Zhang, Zhichao;Zhang, Qi;Li, Kainan;Zhang, Sicong;Shi, Xuechao;Zhao, Yao;Yang, Yuheng;Duan, Kaixuan;Ye, Wenwu;Wang, Yiming;Dong, Suomeng;Wang, Yan;Ma, Zhenchuan;Wang, Yuanchao;Wang, Zhaoyun;Liu, Lin;Liu, Lin

作者机构：

关键词： apoplastic immunity; AlphaFold-guided redesign; broad-spectrum disease resistance; pectin methyl- esterase inhibitor; cell wall

期刊名称：MOLECULAR PLANT （ 影响因子：24.1； 五年影响因子：25.8 ）

ISSN： 1674-2052

年卷期： 2024 年 17 卷 9 期

页码：

收录情况： SCI

摘要： Plant cell walls are a critical site where plants and pathogens continuously struggle for physiological dominance. Here we show that dynamic remodeling of pectin methylesterification of plant cell walls is a component of the physiological and co-evolutionary struggles between hosts and pathogens. A pectin methylesterase (PsPME1) secreted by Phytophthora sojae decreases the degree of pectin methylesterification, thus synergizing with an endo-polygalacturonase (PsPG1) to weaken plant cell walls. To counter PsPME1mediated susceptibility, a plant-derived pectin methylesterase inhibitor protein, GmPMI1, protects pectin to maintain a high methylesterification status. GmPMI1 protects plant cell walls from enzymatic degradation by inhibiting both soybean and P. sojae pectin methylesterases during infection. However, constitutive expression of GmPMI1 disrupted the trade-off between host growth and defense responses. We therefore used AlphaFold structure tools to design a modified form of GmPMI1 (GmPMI1R) that specifically targets and inhibits pectin methylesterases secreted from pathogens but not from plants. Transient expression of GmPMI1R enhanced plant resistance to oomycete and fungal pathogens. In summary, our work highlights the biochemical modification of the cell wall as an important focal point in the physiological and coevolutionary conflict between hosts and microbes, providing an important proof of concept that AIdriven structure-based tools can accelerate the development of new strategies for plant protection.

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