Epigenetic Modification Agents Improve Genomic Methylation Reprogramming in Porcine Cloned Embryos

文献类型： 外文期刊

第一作者： Huan, Yan Jun

作者： Huan, Yan Jun;Wang, Hong Mei;Bin He, Hong;Huan, Yan Jun;Zhu, Jiang;Xie, Bing Teng;Li, Jing Yu;Kong, Qing Ran;Liu, Zhong Hua;Zhu, Jiang;Wu, Zhan Feng;Zhang, Ji Guang

作者机构：

关键词： DNA methylation; Epigenetic modification agents; Pig; Reprogramming; Somatic cell nuclear transfer

期刊名称：JOURNAL OF REPRODUCTION AND DEVELOPMENT （ 影响因子：2.214； 五年影响因子：2.221 ）

ISSN： 0916-8818

年卷期： 2014 年 60 卷 5 期

页码：

收录情况： SCI

摘要： Incomplete DNA methylation reprogramming in cloned embryos leads to low cloning efficiency. Our previous studies showed that the epigenetic modification agents 5-aza-2'-deoxycytidine (5-aza-dC) or trichostatin A (TSA) could enhance the developmental competence of porcine cloned embryos. Here, we investigated genomic methylation dynamics and specific gene expression levels during early embryonic development in pigs. In this study, our results showed that there was a typical wave of DNA demethylation and remethylation of centromeric satellite repeat (CenRep) in fertilized embryos, whereas in cloned embryos, delayed demethylation and a lack of remethylation were observed. When cloned embryos were treated with 5-aza-dC or TSA, CenRep methylation reprogramming was improved, and this was similar to that detected in fertilized counterparts. Furthermore, we found that the epigenetic modification agents, especially TSA, effectively promoted silencing of tissue specific genes and transcription of early embryo development-related genes in porcine cloned embryos. In conclusion, our results showed that the epigenetic modification agent 5-aza-dC or TSA could improve genomic methylation reprogramming in porcine cloned embryos and regulate the appropriate expression levels of genes related to early embryonic development, thereby resulting in high developmental competence.

分类号： S814

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