Protection Evaluation of a Five-Gene-Deleted African Swine Fever Virus Vaccine Candidate Against Homologous Challenge

文献类型： 外文期刊

第一作者： Xie, Zhenhua

作者： Xie, Zhenhua;Liu, Yingnan;Liu, Jingyi;Chen, Zongyan;Li, Yao;Yu, Wanqi;Lv, Lu;Zhong, Qiuping;Song, Yingying;Liao, Xinxin;Chen, Hongjun;Xie, Zhenhua;Liu, Yingnan;Liu, Jingyi;Chen, Zongyan;Li, Yao;Yu, Wanqi;Lv, Lu;Zhong, Qiuping;Song, Yingying;Liao, Xinxin;Chen, Hongjun;Di, Dongdong;Song, Qingqing;Gong, Lang;Wang, Heng

作者机构：

关键词： African swine fever virus; recombinant viruses; live attenuated vaccine; safety; protective efficacy

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：6.064； 五年影响因子：6.843 ）

ISSN：

年卷期： 2022 年 13 卷

页码：

收录情况： SCI

摘要： African swine fever virus (ASFV) represents a serious threat to the global swine industry, and there are no safe or commercially available vaccines. Previous studies have demonstrated that inactivated vaccines do not provide sufficient protection against ASFV and that attenuated vaccines are effective, but raise safety concerns. Here, we first constructed a deletion mutant in which EP153R and EP402R gene clusters were knocked out. Based on the deletion mutant, a further deletion from the MGF_360-12L, MGF_360-13L to MGF_360-14L genes was obtained. The five-genes knockout virus was designated as ASFV-Delta ECM3. To investigate the efficacy and safety of the ASFV-Delta ECM3 virus as a vaccine candidate, the evaluation of the virus was subsequently carried out in pigs. The results showed that the ASFV-Delta ECM3 virus could induce homologous protection against the parental isolate, and no significant clinical signs or viremia were observed. These results show that the contiguous deletion mutant, ASFV-Delta ECM3 encompassing the EP153R/EP402R and MGF_360-12L/13L/14L genes, could be a potential live-attenuated vaccine candidate for the prevention of ASFV infection.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序