High-quality wild barley genome assemblies and annotation with Nanopore long reads and Hi-C sequencing data

文献类型： 外文期刊

第一作者： Pan, Rui

作者： Pan, Rui;Xu, Le;Xu, Yanhao;Zhang, Wenying;Hu, Haifei;Li, Chengdao;He, Tianhua;Hu, Haifei;Hu, Haifei;Hu, Haifei;Hu, Haifei;Xiao, Yuhui;Ouyang, Kai;Xu, Le;Xu, Yanhao;Li, Chengdao;Zhang, Wenying

作者机构：

期刊名称：SCIENTIFIC DATA （ 影响因子：9.8； 五年影响因子：10.8 ）

ISSN：

年卷期： 2023 年 10 卷 1 期

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收录情况： SCI

摘要： Wild barley, from "Evolution Canyon (EC)" in Mount Carmel, Israel, are ideal models for cereal chromosome evolution studies. Here, the wild barley EC_S1 is from the south slope with higher daily temperatures and drought, while EC_N1 is from the north slope with a cooler climate and higher relative humidity, which results in a differentiated selection due to contrasting environments. We assembled a 5.03 Gb genome with contig N50 of 3.53 Mb for wild barley EC_S1 and a 5.05 Gb genome with contig N50 of 3.45 Mb for EC_N1 using 145 Gb and 160.0 Gb Illumina sequencing data, 295.6 Gb and 285.35 Gb Nanopore sequencing data and 555.1 Gb and 514.5 Gb Hi-C sequencing data, respectively. BUSCOs and CEGMA evaluation suggested highly complete assemblies. Using full-length transcriptome data, we predicted 39,179 and 38,373 high-confidence genes in EC_S1 and EC_N1, in which 93.6% and 95.2% were functionally annotated, respectively. We annotated repetitive elements and non-coding RNAs. These two wild barley genome assemblies will provide a rich gene pool for domesticated barley.

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