Mucin alleviates colonic barrier dysfunction by promoting spermine accumulation through enhanced arginine metabolism in Limosilactobacillus mucosae

文献类型： 外文期刊

第一作者： Zhou, Xingjian

作者： Zhou, Xingjian;Xu, Qian;Zhang, Xiangyu;Wang, Hao;Bai, Yu;Wu, Yujun;Liu, Xiaoyi;Wang, Zhenyu;Hu, Jie;Huang, Mingyi;Zhao, Jinbiao;Wang, Junjun;Han, Dandan;Pi, Yu

作者机构：

关键词： mucin; gut barrier function; Limosilactobacillus mucosae; spermine; arginine metabolism

期刊名称：MSYSTEMS （ 影响因子：6.4； 五年影响因子：7.3 ）

ISSN： 2379-5077

年卷期： 2024 年 9 卷 5 期

页码：

收录情况： SCI

摘要： Dietary fiber deprivation is linked to probiotic extinction, mucus barrier dysbiosis, and the overgrowth of mucin-degrading bacteria. However, whether and how mucin could rescue fiber deprivation-induced intestinal barrier defects remains largely unexplored. Here, we sought to investigate the potential role and mechanism by which exogenous mucin maintains the gut barrier function. The results showed that dietary mucin alleviated fiber deprivation-induced disruption of colonic barrier integrity and reduced spermine production in vivo. Importantly, we highlighted that microbial-derived spermine production, but not host-produced spermine, increased significantly after mucin supplementation, with a positive association with upgraded colonic Lactobacillus abundance. After employing an in vitro model, the microbial-derived spermine was consistently dominated by both mucin and Lactobacillus spp. Furthermore, Limosilactobacillus mucosae was identified as an essential spermine-producing Lactobacillus spp., and this isolated strain was responsible for spermine accumulation, especially after adhering to mucin in vitro. Specifically, the mucin-supplemented bacterial supernatant of Limosilactobacillus mucosae was verified to promote intestinal barrier functions through the increased spermine production with a dependence on enhanced arginine metabolism. Overall, these findings collectively provide evidence that mucin-modulated microbial arginine metabolism bridged the interplay between microbes and gut barrier function, illustrating possible implications for host gut health.

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