Ad-VT causes ovarian cancer A2780 cell death via mitochondrial apoptosis and autophagy pathways

文献类型： 外文期刊

第一作者： Cui, Yingli

作者： Cui, Yingli;Yue, Ying;Chen, Xin;Li, Wenjie;Li, Shanzhi;Jin, Ningyi;Li, Xiao;Li, Shanzhi;Jin, Ningyi;Li, Xiao;Li, Yiquan;Jin, Ningyi

作者机构：

关键词： Recombinant adenovirus; Apoptin; Ovarian cancer; Apoptosis; Autophagy

期刊名称：TRANSLATIONAL ONCOLOGY （ 影响因子：4.5； 五年影响因子：4.2 ）

ISSN： 1936-5233

年卷期： 2024 年 48 卷

页码：

收录情况： SCI

摘要： Objective: The recombinant adenovirus Ad-apoptin-hTERTp-E1a (Ad-VT) to have a bi-specific oncolytic character in many tumor cells, but its action pathway in killing tumor cells has not been accurately elucidated. Here, we studied the mechanism of apoptosis and autophagy induced by Ad-VT and the interaction between autophagy and apoptosis. Methods: Crystal Violet staining and CCK-8 assays were used to detect the inhibitory effect of Ad-VT on ovarian cancer cells. The antitumor effect of Ad-VT in vivo was analyzed by tumor bearing nude mouse model. Subsequently, flow cytometry and fluorescence staining were used to analyze the main types of apoptosis and autophagy induced by Ad-VT. Results: In this study, through the in vitro cell inhibition assays, we found that Ad-VT has a significant inhibitory effect on ovarian cancer A2780 cells, but no significant inhibitory effect on normal ovarian epithelial cells. Then in vivo experiments showed that Ad-VT significantly inhibited tumor growth and extended the survival time of mice. Subsequent detection of the level of apoptosis found that Ad-VT can cause a strong apoptotic response and kill cells mainly through the endogenous apoptotic pathway. Through the staining analysis of LC3 and the analysis of autophagy-related proteins, it was found that Ad-VT could significantly increase the level of autophagy in A2780 cells, and this was a protective mechanism. Conclusions: Ad-VT, which replicates under the control of the hTERT promoter and expresses apoptin protein, have significant inhibitory effect on ovarian cancer A2780 cells and promote their apoptosis and autophagy.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序