Rapid identification of Amanita citrinoannulata poisoning using colorimetric and real-time fluorescence and loop-mediated isothermal amplification (LAMP) based on the nuclear ITS region

文献类型： 外文期刊

第一作者： Gao, Jie

作者： Gao, Jie;Xie, Ruibin;Wang, Nan;Zhang, Juan;Sun, Xiaoyun;Chen, Ailiang;Gao, Jie;Wang, Hongjing;Tan, Jianxin

作者机构：

关键词： ITS region; Loop-mediated isothermal amplification (LAMP); Authentication; Amanita citrinoannulata

期刊名称：FOOD CHEMISTRY: MOLECULAR SCIENCES （ 影响因子：3.3； 五年影响因子：3.3 ）

ISSN： 2666-5662

年卷期： 2022 年 4 卷

页码：

收录情况： SCI

摘要： Health concerns and financial losses caused by mushroom poisoning have been reported worldwide. Amanita citrinoannulata, a poisonous mushroom commonly found in China, results in a toxic reaction in humans after mistaken ingestion. To reduce the mistaken ingestion of poisonous mushrooms and to improve clinical diagnosis of mushroom poisoning, a rapid mushroom species identification method is required. Such identification methods could be advantageous in the identification of other poisonous mushroom species. This study developed two rapid and sensitive methods for the detection of A. citrinoannulata utilizing colorimetric and real-time loop-mediated isothermal amplification (LAMP) technology and specifically designed primers for the internal transcribed spacer (ITS) genes of A. citrinoannulata. The methods demonstrated high sensitivity as 0.2 ng of A. citrinoannulata DNA could be detected, with no cross-reaction with 41 non-target mushroom species. The entire detection process could be completed within 40 min without requiring complex instruments and can be observed by the naked eye. Therefore, these novel methods can be used for the identification of fresh and cooked mushroom samples and vomit samples, which contain only 1% A. citrinoannulata. Furthermore, these methods facilitate the detection of mushroom poisoning, and thus, have potential to reduce the number of mushroom poisoning-related deaths worldwide.

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