QTL-seq and QTL mapping identify a new locus for Cercospora leaf spot (Cercospora canescens) resistance in mungbean (Vigna radiata) and a cluster of Receptor-like protein 12 (RLP12) genes as candidate genes for the resistance

文献类型： 外文期刊

第一作者： Srichan, Makawan

作者： Srichan, Makawan;Laosatit, Kularb;Somta, Prakit;Srichan, Makawan;Lin, Yun;Yuan, Xingxing;Chen, Xin

作者机构：

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：4.2； 五年影响因子：4.9 ）

ISSN： 0040-5752

年卷期： 2024 年 137 卷 12 期

页码：

收录情况： SCI

摘要： Key messageQTL-seq, linkage mapping, and whole-genome resequencing revealed a new locus (qCLS5.1) controlling Cercospora canescens resistance in mungbean and Receptor-like protein 12 (RLP12) genes as candidate genes for the resistance.AbstractCercospora leaf spot (CLS) disease, caused by Cercospora canescens, is a common disease of mungbean (Vigna radiata). In this study, the genetics of CLS resistance was investigated in a new source of resistance (accession V2817) and the resistance was finely mapped to identify candidate genes. F2 and F2:3 populations of the cross V1197 (susceptible) x V2718 and a BC1F1 population of the cross V1197 x (V1197 x V2817) were used in this study. Segregation analysis suggested that the resistance is controlled by a single dominant gene. QTL-seq using F2 individuals revealed that a single QTL (designated qCLS5.1) on chromosome 5 controlled the resistance. The qCLS5.1 was confirmed in the F2:3 and BC1F1 populations by QTL analysis. Fine mapping using 978 F2 individuals localized qCLS5.1 to a 48.94 Kb region containing three tandemly duplicated Receptor-like protein 12 (RLP12) genes. Whole-genome resequencing and alignment of V1197 and V2817 revealed polymorphisms causing amino acid changes and premature stop codons in the three RLP12 genes. Collectively, these results show that qCLS5.1 is a new locus for CLS resistance in mungbean, and a cluster of RLP12 genes are candidate genes for the resistance. The new locus qCLS5.1 will be useful for molecular breeding of durable CLS-resistant mungbean cultivars.

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