Mn-XRN1 Has an Inhibitory Effect on Ovarian Reproduction in Macrobrachium nipponense

文献类型： 外文期刊

第一作者： Chen, Tianyong

作者： Chen, Tianyong;Fu, Hongtuo;Yuan, Huwei;Qiao, Hui;Jiang, Sufei;Zhang, Wenyi;Xiong, Yiwei;Fu, Hongtuo;Jin, Shubo

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关键词： Macrobrachium nipponense; XRN1; ovarian reproduction; VASA; metamorphosis

期刊名称：GENES （ 影响因子：3.5； 五年影响因子：3.9 ）

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年卷期： 2023 年 14 卷 7 期

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收录情况： SCI

摘要： XRN1 is an exoribonuclease that degrades mRNA in the cytoplasm along the 5 & PRIME;-3 & PRIME; direction. A previous study indicated that it may be involved in the reproduction of Macrobrachium nipponense. Quantitative real-time PCR was used to detect the spatiotemporal expression pattern of Mn-XRN1. At the tissue level, Mn-XRN1 was significantly expressed in the ovary. During development, Mn-XRN1 was significantly expressed at the CS stage of the embryo, on the 10th day post-larval and in the O2 stage of ovarian reproduction. The in situ hybridization results showed the location of Mn-XRN1 in the ovary. The expression of Mn-VASA was significantly increased after in vivo injection of Mn-XRN1 dsRNA. This suggests that Mn-XRN1 negatively regulates the expression of Mn-VASA. Furthermore, we counted the number of M. nipponense at various stages of ovarian reproduction on different days after RNAi. The results showed that ovarian development was significantly accelerated. In general, the results of the present study indicate that Mn-XRN1 has an inhibitory effect on the ovarian maturation of M. nipponense. The inhibitory effect might be through negative regulation of Mn-VASA.

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