NDV-induced autophagy enhances inflammation through NLRP3/Caspase-1 inflammasomes and the p38/MAPK pathway

文献类型： 外文期刊

第一作者： Cai, Juncheng

作者： Cai, Juncheng;Wang, Siyuan;Du, Haoyun;Fan, Lei;Yuan, WeiFeng;Xu, Qiufan;Ren, Jinlian;Lin, Qiuyan;Ren, Tao;Chen, Libin;Cai, Juncheng;Wang, Siyuan;Du, Haoyun;Fan, Lei;Yuan, WeiFeng;Xu, Qiufan;Ren, Jinlian;Lin, Qiuyan;Ren, Tao;Chen, Libin;Cai, Juncheng;Wang, Siyuan;Du, Haoyun;Fan, Lei;Yuan, WeiFeng;Xu, Qiufan;Ren, Jinlian;Lin, Qiuyan;Ren, Tao;Chen, Libin;Cai, Juncheng;Wang, Siyuan;Du, Haoyun;Fan, Lei;Yuan, WeiFeng;Xu, Qiufan;Ren, Jinlian;Lin, Qiuyan;Ren, Tao;Chen, Libin;Xiang, Bin;Ding, Chan

作者机构：

关键词： Autophagy; inflammation; mitochondrial damage; mitophagy; Newcastle disease virus

期刊名称：VETERINARY RESEARCH （ 影响因子：4.4； 五年影响因子：4.3 ）

ISSN： 0928-4249

年卷期： 2023 年 54 卷 1 期

页码：

收录情况： SCI

摘要： Newcastle disease (ND), caused by the Newcastle disease virus (NDV), is a highly virulent infectious disease of poultry. Virulent NDV can cause severe autophagy and inflammation in host cells. While studies have shown a mutual regulatory relationship between autophagy and inflammation, this relationship in NDV infection remains unclear. This study confirmed that NDV infection could trigger autophagy in DF-1 cells to promote cytopathic and viral replication. NDV-induced autophagy was positively correlated with the mRNA levels of inflammatory cytokines such as IL-1 beta, IL-8, IL-18, CCL-5, and TNF-alpha, suggesting that NDV-induced autophagy promotes the expression of inflammatory cytokines. Further investigation demonstrated that NLRP3 protein expression, Caspase-1 activity, and p38 phosphorylation level positively correlated with autophagy, suggesting that NDV-induced autophagy could promote the expression of inflammatory cytokines through NLRP3/Caspase-1 inflammasomes and p38/MAPK pathway. In addition, NDV infection also triggered mitochondrial damage and mitophagy in DF-1 cells, but did not result in a large leakage of reactive oxygen species (ROS) and mitochondrial DNA (mtDNA), indicating that mitochondrial damage and mitophagy do not contribute to the inflammation response during NDV infection.

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