Isoprenylation modification is required for HIPP1-mediated powdery mildew resistance in wheat

文献类型： 外文期刊

第一作者： Wang, Zongkuan

作者： Wang, Zongkuan;Zhang, Heng;Li, Yingbo;Chen, Yiming;Tang, Xiong;Zhao, Jia;Yu, Feifei;Wang, Haiyan;Xiao, Jin;Liu, Jia;Zhang, Xu;Sun, Li;Xie, Qi;Wang, Xiue;Wang, Zongkuan;Zhang, Heng;Li, Yingbo;Zhang, Heng;Li, Yingbo

作者机构：

关键词： heavy-metal-associated protein; plant defence signalling; protein isoprenylation modification; Triticum aestivum L

期刊名称：PLANT CELL AND ENVIRONMENT （ 影响因子：7.947； 五年影响因子：8.452 ）

ISSN： 0140-7791

年卷期： 2023 年 46 卷 1 期

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收录情况： SCI

摘要： Powdery mildew (Pm), caused by Blumeria graminis f.sp. tritici (Bgt), is one of the most important wheat diseases. Heavy-metal-associated isoprenylated plant protein (HIPP1) has been proved playing important roles in response to biotic and a biotic stress. In present study, we proved HIPP1-V from Haynalidia villosa is a positive regulator in Pm resistance. HIPP1-V was rapidly induced by Bgt. Transiently or stably heterologous overexpressing HIPP1-V in wheat suppressed the haustorium formation and enhanced Pm resistance. HIPP1-V isoprenylation was critical for plasma membrane (PM) localization, interaction with E3-ligase CMPG1-V and function in Pm resistance. Bgt infection recruited the isoprenylated HIPP1-V and CMPG1s on PM; blocking the HIPP1 isoprenylation reduced such recruitment and compromised the resistance of OE-CMPG1-V and OE-HIPP1-V. Overexpressing HIPP1-VC148G could not enhance Pm resistance. These indicated the Pm resistance was dependent on HIPP1-V's isoprenylation. DGEs related to the ROS and SA pathways were remarkably enriched in OE-HIPP1-V, revealing their involvement in Pm resistance. Our results provide evidence on the important role of protein isoprenylation in plant defense.

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