Stress tolerance and characterization of biofilm-like probiotic bacteria encapsulated in calcium pectin beads

文献类型： 外文期刊

第一作者： Liu, Lei

作者： Liu, Lei;Deng, Jia;Guo, Shuyu;Li, Yingfeng;Wang, Luya;Liu, Yuanyuan;Xv, Pingkang;Rao, Yu;Liu, Lei;Guo, Shuyu

作者机构：

关键词： Probiotics; Biofilm; Calcium pectin beads; Tolerance; Physicochemical properties

期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （ 影响因子：6.0； 五年影响因子：6.0 ）

ISSN： 0023-6438

年卷期： 2023 年 184 卷

页码：

收录情况： SCI

摘要： Probiotics can regulate gut microbes to maintain human health. However, probiotics are sensitive to environmental conditions, which reduces their survival rates. There are two forms of bacterial cells: single (planktonic) cells and biofilms. Many investigations have verified that biofilm-formed probiotic strains are more resistant to adverse environments than their planktonic counterparts. This study successfully produced high-density pro biotic microcapsules in a biofilm state by preparing probiotic cells in biofilm mode and combining them with microencapsulation techniques (>10 log CFU/g). Lactiplantibacillus paraplantarum L-ZS9 was cultured within calcium pectin beads (CPB) to create CPB-Biofilm-situ. Subsequent tolerance experiments revealed that the CPBBiofilm-situ was highly resistant to acid, the gastrointestinal tract (GIT) environment, and freeze-drying. Furthermore, scanning electron microscopy (SEM) revealed that the cells in CPB-Biofilm-situ displayed a marginalization tendency for the culture. The water content of the beads was approximately 10%, which was deemed acceptable, and the texture characteristics were also determined. The physicochemical properties and structure of the beads were investigated using Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA). This work is a valuable reference for a new generation of probiotic agents in the biofilm state.

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