CRISPR/Cas9-Mediated Knockout of the PxJHBP Gene Resulted in Increased Susceptibility to Bt Cry1Ac Protoxin and Reduced Lifespan and Spawning Rates in Plutella xylostella
文献类型： 外文期刊
第一作者： You, Shijun
作者： You, Shijun;Yao, Shuyuan;Chen, Xuanhao;Hou, Qing;Lei, Gaoke;You, Minsheng;Liu, Yuanyuan;You, Shijun;Yao, Shuyuan;Chen, Xuanhao;Hou, Qing;Lei, Gaoke;You, Minsheng;You, Shijun;Yao, Shuyuan;Chen, Xuanhao;Hou, Qing;Lei, Gaoke;You, Minsheng;Xiong, Lei;Xiong, Lei;Liu, Zhaoxia;Xie, Xuefeng;Liang, Zhengjiao;Yuchi, Zhiguang
作者机构：
关键词： Plutella xylostella; PxJHBP; Cry1Ac; CRISPR/Cas9; life tables
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）
ISSN： 0021-8561
年卷期： 2024 年 72 卷 14 期
页码：
收录情况： SCI
摘要： Juvenile hormone binding protein (JHBP) is a key regulator of JH signaling, and crosstalk between JH and 20-hydroxyecdysone (20E) can activate and fine-tune the mitogen-activated protein kinase cascade, leading to resistance to insecticidal proteins from Bacillis thuringiensis (Bt). However, the involvement of JHBP in the Bt Cry1Ac resistance of Plutella xylostella remains unclear. Here, we cloned a full-length cDNA encoding JHBP, and quantitative real-time PCR (qPCR) analysis showed that the expression of the PxJHBP gene in the midgut of the Cry1Ac-susceptible strain was significantly higher than that of the Cry1Ac-resistant strain. Furthermore, CRISPR/Cas9-mediated knockout of the PxJHBP gene significantly increased Cry1Ac susceptibility, resulting in a significantly shorter lifespan and reduced fertility. These results demonstrate that PxJHBP plays a critical role in the resistance to Cry1Ac protoxin and in the regulation of physiological metabolic processes associated with reproduction in adult females, providing valuable insights to improve management strategies of P. xylostella.
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